Cuevas, BelénArroba, Ana I.de los Reyes, CarolinaGómez-Jaramillo, LauraGonzález-Montelongo, M. CarmenZubía, Eva2022-12-162022-12-162021-11-27Cuevas B, Arroba AI, de Los Reyes C, Gómez-Jaramillo L, González-Montelongo MC, Zubía E. Diterpenoids from the Brown Alga Rugulopteryx okamurae and Their Anti-Inflammatory Activity. Mar Drugs. 2021 Nov 27;19(12):677http://hdl.handle.net/10668/4521Brown algae of the Family Dictyotaceae produce an array of structurally diverse terpenoids, whose biomedical potential in the anti-inflammatory area has been scarcely explored. Herein, the chemical study of the alga Rugulopteryx okamurae has led to the isolation of ten new diterpenoids: rugukadiol A (1), rugukamurals A-C (2-4), and ruguloptones A-F (6-10). The structures of the new compounds were established by spectroscopic means. Compound 1 exhibits an unprecedented diterpenoid skeleton featuring a bridged tricyclic undecane system. Compounds 2-10 belong to the secospatane class of diterpenoids and differ by the oxygenated functions that they contain. In anti-inflammatory assays, the new diterpenoid 1 and the secospatanes 5 and 10 significantly inhibited the production of the inflammatory mediator NO in LPS-stimulated microglial cells Bv.2 and macrophage cells RAW 264.7. Moreover, compounds 1 and 5 were found to strongly inhibit the expression of Nos2 and the pro-inflammatory cytokine Il1b in both immune cell lines.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/DiterpenoidsBrown algaeRugulopteryx okamuraeAnti-inflammatoryNitric oxideCytokineMicrogliaMacrophagesInvasive algaeDiterpenosPhaeophytaAlgas marinasAntiinflamatoriosÓxido nítricoCitocinasMacrófagosMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Inflammatory AgentsMedical Subject Headings::Organisms::Organism Forms::Aquatic OrganismsMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Terpenes::DiterpenesMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::MiceMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Structure-Activity RelationshipMedical Subject Headings::Organisms::Eukaryota::Stramenopiles::PhaeophytaMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::MacrophagesMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Inflammatory AgentsMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Free Radicals::Nitric OxideMedical Subject Headings::Anatomy::Cells::Neuroglia::MicrogliaMedical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Cytokinesresearch article34940676open access10.3390/md191206771660-3397PMC8704470