Fernandez-Caso, BelenFernandez-Caballero, Jose AngelChueca, NataliaRojo, Eukenede Salazar, AdolfoGarcia Buey, LuisaCardeñoso, LauraGarcia, Federico2023-01-252023-01-252019-04-01Fernández-Caso B, Fernández-Caballero JÁ, Chueca N, Rojo E, de Salazar A, García Buey L, et al. Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing. Sci Rep. 2019 Jun 25;9(1):9264.http://hdl.handle.net/10668/14175Current HCV genotyping methods may have some limitations in detecting mixed infections. We aimed to determine the accuracy of genotyping and the detection of mixed-genotype infections using the Abbott-RealTime HCV Genotype II assay (Abbott-RT-PCR) in comparison with a Roche-Next Generation Sequencing assay (Roche-NGS). Plasma samples collected from 139 HCV-infected patients tested with Abbott-RT-PCR, 114 with single genotype (GT) and 25 with mixed GTs were genotyped using Roche-NGS. Roche-NGS confirmed all single GTs obtained with Abbott-RT-PCR. One case of Abbott GT 4 was found as GT 1a using Roche-NGS. Genotype 5 was confirmed using Roche-NGS in 75% cases (3 out of 4 cases). Twenty-five patients were identified as having mixed HCVinfections using Abbott-RT-PCR. The concordance between Abbott-RT-PCR and Roche-NGS was 76% (19 out of 25 cases). Three mixed-GT infections identified with the Abbott assay (two (1b + 4); one (1a + 3)) were reported as pure 1b using Roche-NGS. Very divergent results were found for the other three samples. When compared to Roche-NGS, Abbott-RT-PCR has performed excellently for the determination of patients infected with single GTs. For patients that are categorized as having a mixed infection using Abbott-RT-PCR, we recommend an NGS assay as a confirmation test.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/AdultAgedAged, 80 and overCohort StudiesFemaleGenotypeGenotyping TechniquesHepacivirusHepatitis CHigh-Throughput Nucleotide SequencingHumansMaleMiddle AgedReal-Time Polymerase Chain ReactionViral Nonstructural Proteinsresearch article31239457open accessEstudios de cohortesHepacivirusProteínas no estructurales viralesReacción en cadena en tiempo real de la polimerasaSecuenciación de nucleótidos de alto rendimientoTécnicas de genotipaje10.1038/s41598-019-42605-z2045-2322PMC6592891https://www.nature.com/articles/s41598-019-42605-z.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592891/pdf