Sánchez-Calvo, Juan MAlados Arboledas, Juan CRos Vidal, Luisde Francisco, José LLópez Prieto, María D2025-01-072025-01-072021-07-16https://hdl.handle.net/10668/25028To compare the RT-PCR Allplex SARS-CoV-2/FluA/FluB/RSV Assay (Allplex assay) with other methods of detection of VOC B.1.1.7. Suspected and non-suspected cases of VOC B.1.1.7 were defined according to the VirSNiP assay, which detects N501Y and deletion H69-V70. For pre-screening, the Allplex™ and TaqPath assays were used. One hundred and sixteen suspected and 113 non-suspected cases were included. In the suspected cases, the Allplex assay showed N-gene dropout, or delayed Ct values of 6.27 ± 1.21 and 6.66 ± 1.41 compared with those of the RdRP and S-gene target, respectively. Agreement between the Allplex and TaqPath assays was 100% when the RdRP and S-gene targets had Ct valuesen2019-nCoV RT-PCR diagnostic panelMutationSARS-CoV-2Screening AssaysVariantWhole-genome sequencingCOVID-19Coronavirus Nucleocapsid ProteinsFeasibility StudiesGene Expression Regulation, ViralGenome, ViralHumansPhosphoproteinsSARS-CoV-2Sensitivity and SpecificityWhole Genome Sequencingresearch article34464903open access10.1016/j.diagmicrobio.2021.1154911879-0070PMC8282941https://doi.org/10.1016/j.diagmicrobio.2021.115491https://pmc.ncbi.nlm.nih.gov/articles/PMC8282941/pdf