Quiñones-Vico, María I.Fernández-González, AnaPérez-Castejón, ElenaMontero-Vílchez, TrinidadArias-Santiago, Salvador2022-09-302022-09-302021-02-08Quiñones-Vico MI, Fernández-González A, Pérez-Castejón E, Montero-Vílchez T, Arias-Santiago S. Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model. J Clin Med. 2021 Feb 8;10(4):642http://hdl.handle.net/10668/4200Bioengineered artificial skin substitutes (BASS) are the main treatment used in addition to autografts when skin injuries involve a large body surface area. Antiseptic/antibiotic treatment is necessary to prevent infections in the BASS implant area. This study aims to evaluate the effect of antiseptics and antibiotics on cell viability, structural integrity, and epidermal barrier function in BASS based on hyaluronic acid during a 28 day follow-up period. Keratinocytes (KTs) and dermal fibroblasts (DFs) were isolated from skin samples and used to establish BASS. The following antibiotic/antiseptic treatment was applied every 48 h: colistin (1%), chlorhexidine digluconate (1%), sodium chloride (0.02%), and polyhexanide (0.1%). Cell viability (LIVE/DEAD® assay), structural integrity (histological evaluation), and epidermal barrier function (trans-epidermal water loss, (TEWL), Tewameter®) were also evaluated. Cell viability percentage of BASS treated with chlorhexidine digluconate was significantly lower (p ≤ 0.001) than the other antiseptics at day 28. Compared to other treatments, chlorhexidine digluconate and polyhexanide significantly affected the epithelium. No significant differences were found regarding epidermal barrier. These results may be useful for treatment protocols after implantation of BASS in patients and evaluating them in clinical practice. BASS represent a suitable model to test in vitro the impact of different treatments of other skin wounds.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Antiseptic/antibiotic testingBioengineered artificial skin substituteCell viabilityEpidermal barrier functionRegenerative medicineWound healingAntiinfecciosos localesPiel artificialSupervivencia celularCélulas epidérmicasMedicina regenerativaCicatrización de heridasMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Surgical Equipment::Artificial Organs::Skin, ArtificialMedical Subject Headings::Chemicals and Drugs::Polycyclic Compounds::Macrocyclic Compounds::Peptides, Cyclic::Polymyxins::ColistinMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides::Glycosaminoglycans::Hyaluronic AcidMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Sodium Compounds::Sodium ChlorideMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell SurvivalMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Infective Agents, LocalMedical Subject Headings::Anatomy::Cells::Epithelial Cells::KeratinocytesMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::FibroblastsMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial AgentsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Clinical ProtocolsMedical Subject Headings::Anatomy::Tissues::Epitheliumresearch article33567522open access10.3390/jcm100406422077-0383PMC7914612