Polo-Generelo, SalvadorTorres, BelénGuerrero-Martínez, José ACamafeita, EmilioVázquez, JesúsReyes, José CPintor-Toro, José A2023-05-032023-05-032022-09-15http://hdl.handle.net/10668/21445Long non-coding RNAs (lncRNAs) have emerged as key regulators in a wide range of biological processes. Here, we identified a mouse miRNA-host gene lncRNA (lnc-Nr6a1) upregulated early during epithelial-to-mesenchymal transition (EMT). We show that when lncRNA is processed, it gives rise to two abundant polyadenylated isoforms, lnc-Nr6a1-1 and lnc-Nr6a1-2, and a longer non-polyadenylated microprocessor-driven lnc-pri-miRNA containing clustered pre-miR-181a2 and pre-miR-181b2 hairpins. Ectopic expression of the lnc-Nr6a1-1 or lnc-Nr6a1-2 isoform enhanced cell migration and the invasive capacity of the cells, whereas the expression of the isoforms and miR-181a2 and miR-181b2 conferred anoikis resistance. Lnc-Nr6a1 gene deletion resulted in cells with lower adhesion capacity and reduced glycolytic metabolism, which are restored by lnc-Nr6a1-1 isoform expression. We performed identification of direct RNA interacting proteins (iDRIP) to identify proteins interacting directly with the lnc-Nr6a1-1 isoform. We defined a network of interacting proteins, including glycolytic enzymes, desmosome proteins and chaperone proteins; and we demonstrated that the lnc-Nr6a1-1 isoform directly binds and acts as a scaffold molecule for the assembly of ENO1, ALDOA, GAPDH, and PKM glycolytic enzymes, along with LDHA, supporting substrate channeling for efficient glycolysis. Our results unveil a role of Lnc-Nr6a1 as a multifunctional lncRNA acting as a backbone for multiprotein complex formation and primary microRNAs.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/anoikiscell adhesionglycolytic scaffoldlncRNAmiRNA-host genesmicroprocessorresearch article36136852open access10.3390/ncrna80500622311-553XPMC9498520https://www.mdpi.com/2311-553X/8/5/62/pdf?version=1666173823https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498520/pdf