Groussin, LionelHorvath, AneliaJullian, EricBoikos, SosipatrosRene-Corail, FernandeLefebvre, HerveCephise-Velayoudom, Fritz-LineVantyghem, Marie-CristineChanson, PhilippeConte-Devolx, BernardLucas, MiguelGentil, AlfonsoMalchoff, Carl DTissier, FrédériqueCarney, J AidanBertagna, XavierStratakis, Constantine ABertherat, Jérôme2012-04-232012-04-232006-05Groussin L, Horvath A, Jullian E, Boikos S, Rene-Corail F, Lefebvre H, et al. A PRKAR1A mutation associated with primary pigmented nodular adrenocortical disease in 12 kindreds. J Clin Endocrinol Metab. 2006 May;91(5):1943-9.http://hdl.handle.net/10668/388CONTEXT: Primary pigmented nodular adrenocortical disease (PPNAD), a rare cause of corticotropin-independent Cushing syndrome, can be part of Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac myxomas, and endocrine tumors or be isolated (i). Germline PRKAR1A-inactivating mutations have been observed in both CNC and iPPNAD, but with no apparent genotype-phenotype correlation. OBJECTIVE:The objectives of the study were a detailed phenotyping for CNC manifestations in 12 kindreds bearing the same PRKAR1A mutation and a study of the consequences of the mutation and a potential founder effect. DESIGN: The study consisted of descriptive case reports. SETTING: The study was conducted at two referral centers. PATIENTS: The patients described in this study were referred for PRKAR1A gene mutation analysis because of a diagnosis of apparently iPPNAD. RESULTS: We describe a 6-bp polypyrimidine tract deletion [exon 7 IVS del (-7-->-2)] in 12 unrelated kindreds that were referred for Cushing syndrome due to PPNAD. Nine of the patients had no family history; in two, there was a family history of iPPNAD. Only one patient met the criteria for CNC. Relatives carrying the same mutation had no manifestations of CNC or PPNAD, suggesting a low penetrance of this PRKAR1A defect. A founder effect was excluded by extensive genotyping of chromosome 17 markers. CONCLUSIONS: In conclusion, a small intronic deletion of the PRKAR1A gene is a low-penetrance cause of mainly iPPNAD; it is the first PRKAR1A genetic defect to have an association with a specific phenotype.enmutationdiseaseprimary pigmented nodular adrenocorticalMedical Subject Headings::Diseases::Endocrine System Diseases::Adrenal Gland Diseases::Adrenal Cortex DiseasesMedical Subject Headings::Anatomy::Cells::Cells, CulturedMedical Subject Headings::Diseases::Endocrine System Diseases::Adrenal Gland Diseases::Adrenocortical Hyperfunction::Cushing SyndromeMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Serine-Threonine Kinases::Cyclic Nucleotide-Regulated Protein Kinases::Cyclic AMP-Dependent Protein Kinases::Cyclic AMP-Dependent Protein Kinase Type I::Cyclic AMP-Dependent Protein Kinase RIalpha SubunitMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::Phosphotransferases::Phosphotransferases (Alcohol Group Acceptor)::Protein Kinases::Protein-Serine-Threonine Kinases::Cyclic Nucleotide-Regulated Protein Kinases::Cyclic AMP-Dependent Protein KinasesMedical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Piperidines::Piperidones::CycloheximideMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Founder EffectMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::GenotypeMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messengerresearch article16464939open access10.1210/jc.2005-27080021-972X