RT Journal Article
T1 Clinoptilolite in Dextran Sulphate Sodium-Induced Murine Colitis: Efficacy and Safety of a Microparticulate Preparation.
A1 Nizet, Stephane
A1 Muñoz, Eduardo
A1 Fiebich, Bernd L
A1 Abuja, Peter M
A1 Kashofer, Karl
A1 Zatloukal, Kurt
A1 Tangermann, Simone
A1 Kenner, Lukas
A1 Tschegg, Cornelius
A1 Nagl, Dietmar
A1 Scheichl, Laurenz
A1 Meisslitzer-Ruppitsch, Claudia
A1 Freissmuth, Michael
A1 Berger, Thomas
K1 DSS
K1 colitis
K1 inflammation
K1 zeolite
AB Clinoptilolite is an aluminium silicate of natural origin; the microporous structure and the net negative charge of its crystal lattice allows for adsorption of ions, toxins, inflammatory mediators, and some microorganisms. We generated 2 preparations of purified clinoptilolite, which differed by about 10-fold in particle size, ie, a standard powder (GHC1) and a microparticulate fraction (GHC2) with a size of 3.6 µm and 0.39 µm (d50) respectively. These were examined for their ability to accelerate the recovery of mice from DSS (dextran sulphate sodium)-induced intestinal inflammation. Efficacy of clinoptilolite preparations was investigated by administering DSS-treated mice twice daily with 30 mg GHC2 or GHC1 for 5 consecutive days, followed by 5 days of recovery without DSS. To explore the safety of the microparticulate preparation (GHC2), mice were subjected to 4 cycles of DSS-exposure. We specifically verified that clinoptilolite microparticles were not systemically bioavailable by examining the gut tissue and the liver for the accumulation of microparticles by transmission electron microscopy. Treatment of mice with GHC2 was superior to GHC1 and as effective as the reference compound 5-aminosalicylic acid in ameliorating the damage induced by the exposure to DSS. In addition, no clinoptilolite particle was observed in the intestinal epithelial layer, gut-associated lymph follicles, or in the liver. Our observations confirm that a microparticulate preparation of clinoptilolite is safe and effective in a murine model of inflammatory bowel disease and supports the hypothesis that the adsorptive capacity of clinoptilolite is of potential therapeutic relevance.
PB Oxford University Press
YR 2017
FD 2017-12-19
LK http://hdl.handle.net/10668/11944
UL http://hdl.handle.net/10668/11944
LA en
NO Nizet S, Muñoz E, Fiebich BL, Abuja PM, Kashofer K, Zatloukal K, et al. Clinoptilolite in Dextran Sulphate Sodium-Induced Murine Colitis: Efficacy and Safety of a Microparticulate Preparation. Inflamm Bowel Dis. 2017 Dec 19;24(1):54-66.
DS RISalud
RD Apr 19, 2025