RT Journal Article
T1 Human adipose tissue-derived mesenchymal stromal cells and their phagocytic capacity.
A1 Costela Ruiz, Víctor J
A1 Melguizo Rodríguez, Lucía
A1 Illescas Montes, Rebeca
A1 García Recio, Enrique
A1 Arias Santiago, Salvador
A1 Ruiz, Concepción
A1 De Luna Bertos, Elvira
K1 immunogenicity
K1 infection
K1 phagocytosis
K1 stem cell-microenvironment interactions
K1 stromal cells
AB Mesenchymal stromal cells (MSCs) have evidenced considerable therapeutic potential in numerous clinical fields, especially in tissue regeneration. The immunological characteristics of this cell population include the expression of Toll-like receptors and mannose receptors, among others. The study objective was to determine whether MSCs have phagocytic capacity against different target particles. We isolated and characterized three human adipose tissue MSC (HAT-MSC) lines from three patients and analysed their phagocytic capacity by flow cytometry, using fluorescent latex beads, and by transmission electron microscopy, using Escherichia coli, Staphylococcus aureus and Candida albicans as biological materials and latex beads as non-biological material. The results demonstrate that HAT-MSCs can phagocyte particles of different nature and size. The percentage of phagocytic cells ranged between 33.8% and 56.2% (mean of 44.37% ± 11.253) according to the cell line, and a high phagocytic index was observed. The high phagocytic capacity observed in MSCs, which have known regenerative potential, may offer an advance in the approach to certain local and systemic infections.
YR 2021
FD 2021-12-02
LK http://hdl.handle.net/10668/19984
UL http://hdl.handle.net/10668/19984
LA en
DS RISalud
RD Apr 10, 2025