TY  - JOUR
AU  - Sánchez-Hernández, Sabina
AU  - Aguilar-González, Araceli
AU  - Guijarro-Albaladejo, Beatriz
AU  - Maldonado-Pérez, Noelia
AU  - Ramos-Hernández, Iris
AU  - Cortijo-Gutiérrez, Marina
AU  - Sánchez Martín, Rosario María
AU  - Benabdellah, Karim
AU  - Martin, Francisco
PY  - 2020
DO  - 10.3390/cells9061492
UR  - http://hdl.handle.net/10668/3877
T2  - Cells
AB  - In spite of the enormous potential of CRISPR/Cas in basic and applied science, the levels of undesired genomic modifications cells still remain mostly unknown and controversial. Nowadays, the efficiency and specificity of the cuts generated by...
LA  - en
PB  - MDPI
KW  - Models
KW  - DNA donor
KW  - On-target integration
KW  - Homologous directed recombination (HDR)
KW  - Efficacy
KW  - Safety
KW  - Specificity
KW  - eGFP
KW  - dsRED
KW  - CRISPR/Cas9
KW  - Modelos estructurales
KW  - Reparación del ADN por recombinación
KW  - Eficacia
KW  - Seguridad
KW  - Especificidad
KW  - Proteínas fluorescentes verdes
KW  - Proteína 9 asociada a CRISPR
KW  - ADN recombinante
KW  - Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Recombinant
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Gene Targeting
KW  - Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Genes, Reporter
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Genetic Engineering
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Genetic Therapy
KW  - Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genetic Vectors
KW  - Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
KW  - Medical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor::K562 Cells
KW  - Medical Subject Headings::Organisms::Viruses::RNA Viruses::Retroviridae::Lentivirus
KW  - Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Microfilament Proteins::Wiskott-Aldrich Syndrome Protein Family::Wiskott-Aldrich Syndrome Protein
KW  - Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Epigenesis, Genetic::Gene Silencing::CRISPR-Cas Systems
KW  - Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Recombination, Genetic::Homologous Recombination
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Theoretical::Models, Biological::Models, Genetic
TI  - Development of Cellular Models to Study Efficiency and Safety of Gene Edition by Homologous Directed Recombination Using the CRISPR/Cas9 System
TY  - research article
ER  -