RT Journal Article
T1 Processing of DNA Polymerase-Blocking Lesions during Genome Replication Is Spatially and Temporally Segregated from Replication Forks.
A1 Wong, Ronald P
A1 García-Rodríguez, Néstor
A1 Zilio, Nicola
A1 Hanulová, Mária
A1 Ulrich, Helle D
K1 DNA damage bypass
K1 DNA replication
K1 DNA replication stress
K1 PCNA
K1 RAD6 pathway
K1 RPA
K1 daughter-strand gaps
K1 homologous recombination
K1 translesion synthesis
K1 ubiquitin
AB Tracing DNA repair factors by fluorescence microscopy provides valuable information about how DNA damage processing is orchestrated within cells. Most repair pathways involve single-stranded DNA (ssDNA), making replication protein A (RPA) a hallmark of DNA damage and replication stress. RPA foci emerging during S phase in response to tolerable loads of polymerase-blocking lesions are generally thought to indicate stalled replication intermediates. We now report that in budding yeast they predominantly form far away from sites of ongoing replication, and they do not overlap with any of the repair centers associated with collapsed replication forks or double-strand breaks. Instead, they represent sites of postreplicative DNA damage bypass involving translesion synthesis and homologous recombination. We propose that most RPA and recombination foci induced by polymerase-blocking lesions in the replication template are clusters of repair tracts arising from replication centers by polymerase re-priming and subsequent expansion of daughter-strand gaps over the course of S phase.
YR 2019
FD 2019-10-10
LK https://hdl.handle.net/10668/28356
UL https://hdl.handle.net/10668/28356
LA en
DS RISalud
RD Apr 4, 2025