RT Journal Article
T1 The CoQH2/CoQ Ratio Serves as a Sensor of Respiratory Chain Efficiency.
A1 Guarás, Adela
A1 Perales-Clemente, Ester
A1 Calvo, Enrique
A1 Acín-Pérez, Rebeca
A1 Loureiro-Lopez, Marta
A1 Pujol, Claire
A1 Martínez-Carrascoso, Isabel
A1 Nuñez, Estefanía
A1 García-Marqués, Fernando
A1 Rodríguez-Hernández, María Angeles
A1 Cortés, Ana
A1 Diaz, Francisca
A1 Pérez-Martos, Acisclo
A1 Moraes, Carlos T
A1 Fernández-Silva, Patricio
A1 Trifunovic, Aleksandra
A1 Navas, Plácido
A1 Vazquez, Jesús
A1 Enríquez, Jose A
AB Electrons feed into the mitochondrial electron transport chain (mETC) from NAD- or FAD-dependent enzymes. A shift from glucose to fatty acids increases electron flux through FAD, which can saturate the oxidation capacity of the dedicated coenzyme Q (CoQ) pool and result in the generation of reactive oxygen species. To prevent this, the mETC superstructure can be reconfigured through the degradation of respiratory complex I, liberating associated complex III to increase electron flux via FAD at the expense of NAD. Here, we demonstrate that this adaptation is driven by the ratio of reduced to oxidized CoQ. Saturation of CoQ oxidation capacity induces reverse electron transport from reduced CoQ to complex I, and the resulting local generation of superoxide oxidizes specific complex I proteins, triggering their degradation and the disintegration of the complex. Thus, CoQ redox status acts as a metabolic sensor that fine-tunes mETC configuration in order to match the prevailing substrate profile.
YR 2016
FD 2016-03-24
LK http://hdl.handle.net/10668/9974
UL http://hdl.handle.net/10668/9974
LA en
DS RISalud
RD Apr 4, 2025