RT Journal Article
T1 Effective anti-leishmanial activity of minimalist squaramide-based compounds.
A1 Marín, Clotilde
A1 Ximenis, Marta
A1 Ramirez-Macías, Inmaculada
A1 Rotger, Carmen
A1 Urbanova, Kristina
A1 Olmo, Francisco
A1 Martín-Escolano, Rubén
A1 Rosales, María José
A1 Cañas, Rocio
A1 Gutierrez-Sánchez, Ramón
A1 Costa, Antonio
A1 Sánchez-Moreno, Manuel
K1 L. braziliensis
K1 L. donovani
K1 Leishmania infantum
K1 Metabolite excretion
K1 Squaramides
K1 Ultrastructural alterations
AB In order to evaluate the in vitro leishmanicidal activity of N,N'-Squaramides derivatives, compounds that feature both hydrogen bond donor and acceptor groups and are capable of multiple interactions with complementary sites, against Leishmania infantum, Leishmania braziliensis and Leishmania donovani a series of 18compounds was prepared and assayed on extracellular and intracellular parasite forms. Infectivity and cytotoxicity tests were performed on J774.2 macrophage cells using meglumine antimoniate (Glucantime) as the reference drug. Changes in metabolite excretion by 1H-NMR and the ultrastructural alterations occurring in the parasites treated using transmission electron microscopy (TEM), was analyzed. Compounds 1, 7, 11, 14 and 17 were the more active and less toxic. Infection rates showed that the order of effectiveness was 17 > 11 > 14 > 7 for both L. infantum and L. braziliensis and in the same way, the compound 1 for L. donovani. All these compounds have altered the typical structure of the promastigotes, glycosomes and mitochondria. These severe modifications by the compounds are the ultimate reasons for the alterations observed in the excretion products. The Squaramide 17 (3-(butylamino)-4-((3-(dimetilamino)propyl)(methyl)amino)cyclobut-3-en-1,2-dione) was clearly the most efficient of all compounds. The data appear to confirm that the severe modifications generated in organelles such as glycosomes or mitochondria by the compounds are the ultimate reasons for the alterations observed in the excretion products of all species. The activity, stability, low cost of starting materials, and straightforward synthesis make amino squaramides appropriate molecules for the development of an affordable anti-leishmanial agent.
YR 2016
FD 2016-07-30
LK http://hdl.handle.net/10668/10326
UL http://hdl.handle.net/10668/10326
LA en
DS RISalud
RD Apr 11, 2025