RT Journal Article
T1 Melatonin-doped polymeric nanoparticles induce high crystalline apatite formation in root dentin.
A1 Toledano-Osorio, Manuel
A1 Aguilera, Fátima S
A1 Muñoz-Soto, Esther
A1 Osorio, Estrella
A1 Toledano, Manuel
A1 Escames, Germaine
A1 Medina-Castillo, Antonio L
A1 Osorio, María T
A1 López-López, Modesto T
A1 Vallecillo-Rivas, Marta
A1 Osorio, Raquel
K1 Apatite
K1 Dentin
K1 Hardness
K1 Melatonin
K1 Polymeric nanoparticles
K1 Remineralization
K1 Transmission electron microscopy
K1 X-ray diffraction
AB To investigate the effect of novel polymeric nanoparticles (NPs) doped with melatonin (ML) on nano-hardness, crystallinity and ultrastructure of the formed hydroxyapatite after endodontic treatment. Undoped-NPs and ML-doped NPs (ML-NPs) were tested at radicular dentin, after 24 h and 6 m. A control group without NPs was included. Radicular cervical and apical dentin surfaces were studied by nano-hardness measurements, X-ray diffraction and transmission electron microscopy. Mean and standard deviation were analyzed by ANOVA and Student-Newman-Keuls multiple comparisons (p  Cervical dentin treated with undoped NPs maintained its nano-hardness values after 6 m of storage being [24 h: 0.29 (0.01); 6 m: 0.30 (0.02) GPa], but it decreased at apical dentin [24 h: 0.36 (0.01); 6 m: 0.28 (0.02) GPa]. When ML-NPs were used, nano-hardness was similar over time [24h: 0.31 (0.02); 6 m: 0.28 (0.03) GPa], at apical dentin. Root dentin treated with ML-NPs produced, in general, high crystallinity of new minerals and thicker crystals than those produced in the rest of the groups. After 6 m, crystals became organized in randomly oriented polyhedral, square polygonal block-like apatite or drop-like apatite polycrystalline lattices when ML-NPs were used. Undoped NPs generated poor crystallinity, with preferred orientation of small crystallite and increased microstrain. New polycrystalline formations encountered in dentin treated with ML-NPs may produce structural dentin stability and high mechanical performance at the root. The decrease of mechanical properties over time in dentin treated without NPs indicates scarce remineralization potential, dentin demineralization and further potential degradation. The amorphous stage may provide high hydroxyapatite solubility and remineralizing activity.
YR 2021
FD 2021-09-17
LK https://hdl.handle.net/10668/27743
UL https://hdl.handle.net/10668/27743
LA en
DS RISalud
RD Apr 10, 2025