RT Journal Article
T1 The Helicase PIF1 Facilitates Resection over Sequences Prone to Forming G4 Structures.
A1 Jimeno, Sonia
A1 Camarillo, Rosa
A1 Mejías-Navarro, Fernando
A1 Fernández-Ávila, Maria Jesús
A1 Soria-Bretones, Isabel
A1 Prados-Carvajal, Rosario
A1 Huertas, Pablo
K1 BRCA1
K1 DNA end resection
K1 DNA recombination
K1 DNA repair
K1 DSB repair
K1 G-quadruplex
K1 PIF1
AB DNA breaks are complex lesions that can be repaired either by non-homologous end joining (NHEJ) or by homologous recombination (HR). The decision between these two routes of DNA repair is a key point of the DNA damage response (DDR) that is controlled by DNA resection. The core machinery catalyzing the resection process is well established. However, little is known about the additional requirements of DNA resection over DNA structures with high complexity. Here, we found evidence that the human helicase PIF1 has a role in DNA resection, specifically for defined DNA regions, such as those prone to form G-quadruplexes. Indeed, PIF1 is recruited to the site of DNA damage and physically interacts with proteins involved in DNA resection, and its depletion causes DNA damage sensitivity and a reduction of HR efficiency. Moreover, G4 stabilization by itself hampers DNA resection, a phenomenon suppressed by PIF1 overexpression.
YR 2018
FD 2018
LK http://hdl.handle.net/10668/12964
UL http://hdl.handle.net/10668/12964
LA en
DS RISalud
RD Apr 10, 2025