RT Journal Article
T1 Multilocus Genotyping of Pneumocystis jirovecii from Deceased Cuban AIDS Patients Using Formalin-Fixed and Paraffin-Embedded Tissues
A1 Friaza, Vicente
A1 de-Armas, Yaxsier
A1 Capo, Virginia
A1 Morilla, Ruben
A1 Plascencia-Hernandez, Arturo
A1 Perez-Gomez, Hector R.
A1 Iglesias, Enrique
A1 Fonte, Luis
A1 de-la-Horra, Carmen
A1 Calderon, Enrique J.
K1 pneumocystis
K1 genotype
K1 epidemiology
K1 autopsied lungs
K1 Genetic diversity
K1 Carinii
K1 Dna
K1 Polymorphisms
K1 Infection
K1 Diagnosis
K1 Single
AB The results of the genotypic characterization of Pneumocystis jirovecii are described in lung tissue samples from 41 Cubans who died of AIDS with pneumocystosis between 1995 and 2008. Histological sections of the lung preserved as formalin-fixed and paraffin-embedded tissue were examined. PCR amplification and nucleotide sequencing of the two mitochondrial genes (large and small) of the pathogen allowed verification of a predominance of genotype 3 (85T/248C) of the large mitochondrial gene and genotype 3 (160A/196T) of the small mitochondrial gene over a period of 14 years (1995-2008). These results suggest that the 85T/248C//160A/196T genotype circulates with the highest frequency (81.3%) among AIDS patients in Cuba. Multilocus analysis indicates a limited circulation of pathogen genotypes on the island with the existence of a clonal genotype with an epidemic structure. Furthermore, it appears that circulating strains of P. jirovecii have not developed mutations related to sulfonamide resistance. Taken together, the data in this study revealed important elements about pneumocystosis in Cuban patients dying of AIDS and the usefulness of formalin-fixed and paraffin-embedded samples to carry out molecular epidemiology studies of P. jirovecii.
PB Mdpi
YR 2021
FD 2021-12-05
LK https://hdl.handle.net/10668/27847
UL https://hdl.handle.net/10668/27847
LA en
NO Friaza V, de Armas Y, Capó V, Morilla R, Plascencia-Hernández A, Pérez-Gómez HR, et al. Multilocus Genotyping of Pneumocystis jirovecii from Deceased Cuban AIDS Patients Using Formalin-Fixed and Paraffin-Embedded Tissues. J Fungi (Basel). 2021 Dec 5;7(12):1042.
DS RISalud
RD Apr 19, 2025