TY  - JOUR
AU  - Morata, Pilar
AU  - Queipo-Ortuño, María I.
AU  - Reguera, Jose M.
AU  - García-Ordoñez, Miguel A.
AU  - Cárdenas, Ana
AU  - Colmenero, Juan D
PY  - 2003
SN  - 0095-1137
UR  - http://hdl.handle.net/10668/2450
T2  - Journal of Clinical Microbiology
AB  - In order to overcome some of the limitations of conventional microbiological techniques in the diagnosis of human brucellosis, a simple PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed. After amplification of a 223-bp sequence of a gene...
LA  - en
PB  - American Society for Microbiology
KW  - Brucelosis
KW  - Enzimoinmunoanálisis por adsorción
KW  - Humanos
KW  - Reacción en cadena de la polimerasa
KW  - Juego de reactivos para diagnóstico
KW  - Reproducibilidad de los resultados
KW  - Sensibilidad y especificidad
KW  - Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunohistochemistry::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assay
KW  - Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Reagent Kits, Diagnostic
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Evaluation Studies as Topic::Reproducibility of Results
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and Specificity
KW  - Medical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Brucellaceae::Brucella
TI  - Development and evaluation of a PCR-enzyme-linked immunosorbent assay for diagnosis of human brucellosis.
TY  - research article
ER  -