RT Journal Article
T1 Cardiorespiratory fitness in children with overweight/obesity: Insights into the molecular mechanisms.
A1 Plaza-Florido, Abel
A1 Altmäe, Signe
A1 Esteban, Francisco J
A1 Löf, Marie
A1 Radom-Aizik, Shlomit
A1 Ortega, Francisco B
K1 RNA-seq
K1 aerobic fitness
K1 childhood
K1 gene expression
K1 transcriptome
AB High cardiorespiratory fitness (CRF) levels reduce the risk of developing cardiovascular disease (CVD) during adulthood. However, little is known about the molecular mechanisms underlying the health benefits of high CRF levels at the early stage of life. This study aimed to analyze the whole-blood transcriptome profile of fit children with overweight/obesity (OW/OB) compared to unfit children with OW/OB. 27 children with OW/OB (10.14 ± 1.3 years, 59% boys) from the ActiveBrains project were evaluated. VO2 peak was assessed using a gas analyzer, and participants were categorized into fit or unfit according to the CVD risk-related cut-points. Whole-blood transcriptome profile (RNA sequencing) was analyzed. Differential gene expression analysis was performed using the limma R/Bioconductor software package (analyses adjusted by sex and maturational status), and pathways' enrichment analysis was performed with DAVID. In addition, in silico validation data mining was performed using the PHENOPEDIA database. 256 genes were differentially expressed in fit children with OW/OB compared to unfit children with OW/OB after adjusting by sex and maturational status (FDR  The distinct pattern of whole-blood gene expression in fit children with OW/OB reveals genes and gene pathways that might play a role in reducing CVD risk factors later in life.
YR 2021
FD 2021-08-14
LK http://hdl.handle.net/10668/18279
UL http://hdl.handle.net/10668/18279
LA en
DS RISalud
RD Apr 7, 2025