RT Journal Article
T1 A Humanized Diet Profile May Facilitate Colonization and Immune Stimulation in Human Microbiota-Colonized Mice
A1 Moreno-Indias, Isabel
A1 Lundberg, Randi
A1 Krych, Lukasz
A1 Metzdorff, Stine Broeng
A1 Kot, Witold
A1 Sørensen, Dorte Bratbo
A1 Nielsen, Dennis Sandris
A1 Hansen, Camilla Hartmann Friis
A1 Hansen, Axel K.
K1 Gastrointestinal microbiome
K1 Mice
K1 Diet
K1 Fecal microbiota transplantation
K1 Lymphocytes
K1 Flow cytometry
K1 Gene expression
K1 Microbiota
K1 Microbioma gastrointestinal
K1 Ratones
K1 Dieta
K1 Trasplante de microbiota fecal
K1 Linfocitos
K1 Citometría de flujo
K1 Expresión génica
AB Background: In spite of the importance of the use of gnotobiotic mice for human fecal transfer, colonization efficiency and immune stimulation after human microbiota inoculation in mice are poorly studied compared to mouse microbiota inoculation. We tested the colonization efficiency and immune responses in mice bred for one additional generation after inoculating the parent generation with either a human (HM) or a mouse microbiota (MM). Furthermore, we tested if colonization efficiency and immune stimulation could be improved in HM-colonized mice by dietary approaches: if these were fed a diet closer to the human diet either in its sources of animal fat and protein [the “animal source” (AS) diet] or in its proportions of macronutrients from the normal sources of a mouse diet [the “human profile” (HP) diet]. Results: Although significantly lower in mice with a human microbiota (30–40% vs. 61– 70%) the colonization efficiency was significantly higher in HM mice fed the HP diet (40%), and in MM mice fed AS (70%). The microbiota of mice fed HP was comparable to the microbiota of mice fed a standard rodent chow, while the microbiota of mice fed the animal source diet (AS) clustered separately. Mice inoculated with mouse fecal matter had significantly more CD4+ T cells and Cd4 expression and significantly fewer regulatory T cells (Tregs) and FoxP3 expression than human microbiota inoculated mice, but cell proportions differences were mostly apparent between mice fed the AS diet. Mice fed the HP diet had significantly higher expression of Cd8a. Conclusion: It is concluded that a diet with a humanized profile could support the establishment of a human microbiota in mice, which will, however, still elicit a lower colonization efficiency compared to mice inoculated with a mouse microbiota.
PB Frontiers
YR 2020
FD 2020-06-19
LK http://hdl.handle.net/10668/3899
UL http://hdl.handle.net/10668/3899
LA en
NO Moreno-Indias I, Lundberg R, Krych L, Metzdorff SB, Kot W, Sørensen DB, et al. A Humanized Diet Profile May Facilitate Colonization and Immune Stimulation in Human Microbiota-Colonized Mice. Front Microbiol. 2020 Jun 19;11:1336
DS RISalud
RD Apr 12, 2025