RT Journal Article
T1 Laboratory detection of respiratory viruses by automated techniques.
A1 Pérez-Ruiz, Mercedes
A1 Pedrosa-Corral, Irene
A1 Sanbonmatsu-Gámez, Sara
A1 Navarro-Marí, María
K1 Acute respiratory infection
K1 Automation
K1 Influenza-like illness
K1 microarray
K1 Real-time PCR
K1 Respiratory viruses
K1 Infecciones del Sistema Respiratorio
K1 Automatización
K1 Análisis por Micromatrices
K1 Reacción en Cadena en Tiempo Real de la Polimerasa
AB Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.
PB Bentham Open
YR 2012
FD 2012-11-30
LK http://hdl.handle.net/10668/907
UL http://hdl.handle.net/10668/907
LA en
NO Pérez-Ruiz M, Pedrosa-Corral I, Sanbonmatsu-Gámez S, Navarro-Marí M. Laboratory detection of respiratory viruses by automated techniques. Open Virol J 2009; 6:151-9
NO Journal Article; Se utiliza la url que lleva al articulo en pdf porque la url del abstract es incorrecta.
DS RISalud
RD Apr 8, 2025