RT Journal Article
T1 Low adherent cancer cell subpopulations are enriched in tumorigenic and metastatic epithelial-to-mesenchymal transition-induced cancer stem-like cells.
A1 Morata-Tarifa, Cynthia
A1 Jiménez, Gema
A1 García, María A
A1 Entrena, José M
A1 Griñán-Lisón, Carmen
A1 Aguilera, Margarita
A1 Picon-Ruiz, Manuel
A1 Marchal, Juan A
K1 Animales
K1 Índice de masa corporal
K1 Mama
K1 Neoplasias de la mama
K1 Cadherinas
K1 Claudinas
K1 Neoplasias del colon
K1 Transición epitelial-mesenquimal
K1 Xenoinjertos
K1 Ratones
K1 Recurrencia local de neoplasia
K1 Células madre neoplásicas
K1 Fenotipo
K1 Tripsina
K1 Caracoles
K1 Regulación hacia arriba
K1 Vimentina
AB Cancer stem cells are responsible for tumor progression, metastasis, therapy resistance and cancer recurrence, doing their identification and isolation of special relevance. Here we show that low adherent breast and colon cancer cells subpopulations have stem-like properties. Our results demonstrate that trypsin-sensitive (TS) breast and colon cancer cells subpopulations show increased ALDH activity, higher ability to exclude Hoechst 33342, enlarged proportion of cells with a cancer stem-like cell phenotype and are enriched in sphere- and colony-forming cells in vitro. Further studies in MDA-MB-231 breast cancer cells reveal that TS subpopulation expresses higher levels of SLUG, SNAIL, VIMENTIN and N-CADHERIN while show a lack of expression of E-CADHERIN and CLAUDIN, being this profile characteristic of the epithelial-to-mesenchymal transition (EMT). The TS subpopulation shows CXCL10, BMI-1 and OCT4 upregulation, differing also in the expression of several miRNAs involved in EMT and/or cell self-renewal such as miR-34a-5p, miR-34c-5p, miR-21-5p, miR-93-5p and miR-100-5p. Furthermore, in vivo studies in immunocompromised mice demonstrate that MDA-MB-231 TS cells form more and bigger xenograft tumors with shorter latency and have higher metastatic potential. In conclusion, this work presents a new, non-aggressive, easy, inexpensive and reproducible methodology to isolate prospectively cancer stem-like cells for subsequent biological and preclinical studies.
PB Nature Publishing Group
YR 2016
FD 2016-01-11
LK http://hdl.handle.net/10668/2299
UL http://hdl.handle.net/10668/2299
LA en
NO Morata-Tarifa C, Jiménez G, García MA, Entrena JM, Griñán-Lisón C, Aguilera M, et al. Low adherent cancer cell subpopulations are enriched in tumorigenic and metastatic epithelial-to-mesenchymal transition-induced cancer stem-like cells. Sci Rep. 2016; 6:18772
NO Journal Article;
DS RISalud
RD Apr 6, 2025