RT Journal Article
T1 Leptin acts in the carotid bodies to increase minute ventilation during wakefulness and sleep and augment the hypoxic ventilatory response.
A1 Caballero-Eraso, Candela
A1 Shin, Mi-Kyung
A1 Pho, Huy
A1 Kim, Lenise J
A1 Pichard, Luis E
A1 Wu, Zhi-Juan
A1 Gu, Chenjuan
A1 Berger, Slava
A1 Pham, Luu
A1 Yeung, Ho-Yee Bonnie
A1 Shirahata, Machiko
A1 Schwartz, Alan R
A1 Tang, Wan-Yee Winnie
A1 Sham, James S K
A1 Polotsky, Vsevolod Y
K1 Carotid Body
K1 Leptin
K1 Sleep Apnoea
AB Leptin is a potent respiratory stimulant. A long functional isoform of leptin receptor, LepRb , was detected in the carotid body (CB), a key peripheral hypoxia sensor. However, the effect of leptin on minute ventilation (VE ) and the hypoxic ventilatory response (HVR) has not been sufficiently studied. We report that LepRb is present in approximately 74% of the CB glomus cells. Leptin increased carotid sinus nerve activity at baseline and in response to hypoxia in vivo. Subcutaneous infusion of leptin increased VE and HVR in C57BL/6J mice and this effect was abolished by CB denervation. Expression of LepRb in the carotid bodies of LepRb deficient obese db/db mice increased VE during wakefulness and sleep and augmented the HVR. We conclude that leptin acts on LepRb in the CBs to stimulate breathing and HVR, which may protect against sleep disordered breathing in obesity. Leptin is a potent respiratory stimulant. The carotid bodies (CB) express the long functional isoform of leptin receptor, LepRb , but the role of leptin in CB has not been fully elucidated. The objectives of the current study were (1) to examine the effect of subcutaneous leptin infusion on minute ventilation (VE ) and the hypoxic ventilatory response to 10% O2 (HVR) in C57BL/6J mice before and after CB denervation; (2) to express LepRb in CB of LepRb -deficient obese db/db mice and examine its effects on breathing during sleep and wakefulness and on HVR. We found that leptin enhanced carotid sinus nerve activity at baseline and in response to 10% O2 in vivo. In C57BL/6J mice, leptin increased VE from 1.1 to 1.5 mL/min/g during normoxia (P 
YR 2018
FD 2018-11-29
LK http://hdl.handle.net/10668/13028
UL http://hdl.handle.net/10668/13028
LA en
DS RISalud
RD Apr 17, 2025