RT Journal Article
T1 Transcriptional changes in dendritic cells underlying allergen specific induced tolerance in a mouse model.
A1 Nuñez, Rafael
A1 Rodriguez, Maria Jose
A1 Palomares, Francisca
A1 Gomez, Francisca
A1 Jabato, Fernando M
A1 Cordoba-Caballero, Jose
A1 Seoane, Pedro
A1 Losada, Jorge
A1 Rojo, Javier
A1 Torres, Maria Jose
A1 Perkins, James Richard
A1 Mayorga, Cristobalina
AB To investigate food allergy-tolerance mechanisms induced through allergen-specific immunotherapy we used RNA-Sequencing to measure gene expression in lymph-node-derived dendritic cells from Pru p 3-anaphylactic mice after immunotherapy with glycodendropeptides at 2 nM and 5 nM, leading to permanent tolerance and short-term desensitization, respectively. Gene expression was also measured in mice receiving no immunotherapy (anaphylaxis); and in which anaphylaxis could never occur (antigen-only). Compared to anaphylaxis, the antigen-only group showed the greatest number of expression-changes (411), followed by tolerant (186) and desensitized (119). Only 29 genes changed in all groups, including Il12b, Cebpb and Ifngr1. The desensitized group showed enrichment for genes related to chronic inflammatory response, secretory granule, and regulation of interleukin-12 production; the tolerant group showed genes related to cytokine receptor activity and glucocorticoid receptor binding, suggesting distinct pathways for similar outcomes. We identified genes and processes potentially involved in the restoration of long-term tolerance via allergen-specific immunotherapy, representing potential prognostic biomarkers.
YR 2022
FD 2022-02-18
LK http://hdl.handle.net/10668/19583
UL http://hdl.handle.net/10668/19583
LA en
DS RISalud
RD Apr 7, 2025