RT Journal Article T1 Characterizing the Invasive Tumor Front of Aggressive Uterine Adenocarcinoma and Leiomyosarcoma. A1 Sanegre, Sabina A1 Eritja, Nuria A1 de-Andrea, Carlos A1 Diaz-Martin, Juan A1 Diaz-Lagares, Angel A1 Jacome, Maria Amalia A1 Salguero-Aranda, Carmen A1 Garcia Ros, David A1 Davidson, Ben A1 Lopez, Rafel A1 Melero, Ignacio A1 Navarro, Samuel A1 Ramon-Y-Cajal, Santiago A1 de-Alava, Enrique A1 Matias-Guiu, Xavier A1 Noguera, Rosa K1 epigenetic profiles K1 extracellular matrix K1 gene expression K1 immune cells K1 reticular fibers K1 tumor microenvironment K1 tumor-host interface AB The invasive tumor front (the tumor-host interface) is vitally important in malignant cell progression and metastasis. Tumor cell interactions with resident and infiltrating host cells and with the surrounding extracellular matrix and secreted factors ultimately determine the fate of the tumor. Herein we focus on the invasive tumor front, making an in-depth characterization of reticular fiber scaffolding, infiltrating immune cells, gene expression, and epigenetic profiles of classified aggressive primary uterine adenocarcinomas (24 patients) and leiomyosarcomas (11 patients). Sections of formalin-fixed samples before and after microdissection were scanned and studied. Reticular fiber architecture and immune cell infiltration were analyzed by automatized algorithms in colocalized regions of interest. Despite morphometric resemblance between reticular fibers and high presence of macrophages, we found some variance in other immune cell populations and distinctive gene expression and cell adhesion-related methylation signatures. Although no evident overall differences in immune response were detected at the gene expression and methylation level, impaired antimicrobial humoral response might be involved in uterine leiomyosarcoma spread. Similarities found at the invasive tumor front of uterine adenocarcinomas and leiomyosarcomas could facilitate the use of common biomarkers and therapies. Furthermore, molecular and architectural characterization of the invasive front of uterine malignancies may provide additional prognostic information beyond established prognostic factors. PB Frontiers Research Foundation SN 2296-634X YR 2021 FD 2021-06-03 LK http://hdl.handle.net/10668/18024 UL http://hdl.handle.net/10668/18024 LA en NO Sanegre S, Eritja N, de Andrea C, Diaz-Martin J, Diaz-Lagares Á, Jácome MA, et al. Characterizing the Invasive Tumor Front of Aggressive Uterine Adenocarcinoma and Leiomyosarcoma. Front Cell Dev Biol. 2021 Jun 3;9:670185. DS RISalud RD Apr 9, 2025