%0 Journal Article
%A Lima-Cabello, Elena
%A Morales-Santana, Sonia
%A Foley, Rhonda C.
%A Melser, Su
%A Alche, Victor
%A Siddique, Kadambot H. M.
%A Singh, Karam B.
%A Alche, Juan D.
%A Jimenez-Lopez, Jose C.
%T Ex vivo and in vitro assessment of anti-inflammatory activity of seed beta-conglutin proteins from Lupinus angustifolius
%D 2018
%@ 1756-4646
%U http://hdl.handle.net/10668/18817
%X The development of functional food ingredients using legume seed proteins has potential for nutraceutical use. We purified recombinant beta-conglutin proteins (r beta 1 to r beta 4, and r beta 6) from narrow-leafed lupin using affinity-chromatography, and evaluated their anti-inflammatory activity using ex vivo and in vitro systems. r beta 1, r beta 3, and r beta 6 produced lower levels of pro-inflammatory mediators such as nitric oxide (about -34.0-fold in all cases), iNOS mRNA (-7.15, -7.97, -7.41-fold), interleukin 1 beta (-12.05, -11.64, -12.16-fold), chemokine CCL5 (-16.0, -18.0, -19.0-fold), and cytokines including TNF-alpha, INF-gamma, IL-1 beta, IL-2, IL-6, IL-8, and IL-12 (beta 1: -28, -100, -8, -2, -49, -45, -127-fold; beta 3: -22, -400, -9, -3, -33, -10, -2.54-fold; beta 6:-72, -122, -11, -3, -2000, -13, -338-fold).These results suggest that the beta 1, beta 3, and beta 6 conglutins have potential as functional food components in nutraceuticals and that can provide alternative therapies for the prevention and treatment of inflammatory related diseases.
%K Anti-inflammatory proteins
%K beta-conglutins
%K chemokine CCL5
%K IL-1 beta
%K iNOS
%K Lupinus angustifolius
%K Vicilin
%K Islet inflammation
%K Nitric-oxide
%K Cytokines
%K Antioxidant
%K Activation
%K Type-1
%K Alpha
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