RT Journal Article
T1 Natural Killer Cells Improve Hematopoietic Stem Cell Engraftment by Increasing Stem Cell Clonogenicity In Vitro and in a Humanized Mouse Model.
A1 Escobedo-Cousin, Michelle
A1 Jackson, Nicola
A1 Laza-Briviesca, Raquel
A1 Ariza-McNaughton, Linda
A1 Luevano, Martha
A1 Derniame, Sophie
A1 Querol, Sergio
A1 Blundell, Michael
A1 Thrasher, Adrian
A1 Soria, Bernat
A1 Cooper, Nichola
A1 Bonnet, Dominique
A1 Madrigal, Alejandro
A1 Saudemont, Aurore
K1 NK cells
K1 Hematopoietic stem cells
K1 Mouse models
K1 Integrins
K1 T cells
K1 Gene expression
K1 Flow cytometry
K1 Cytotoxic T cells
K1 Células Asesinas Naturales
K1 Células Madre Hematopoyéticas
K1 Células Madre Embrionarias de Ratones
K1 Integrinas
K1 Expresión Génica
K1 Citometría de flujo
AB Cord blood (CB) is increasingly used as a source of hematopoietic stem cells (HSC) for transplantation. Low incidence and severity of graft-versus-host disease (GvHD) and a robust graft-versus-leukemia (GvL) effect are observed following CB transplantation (CBT). However, its main disadvantages are a limited number of HSC per unit, delayed immune reconstitution and a higher incidence of infection. Unmanipulated grafts contain accessory cells that may facilitate HSC engraftment. Therefore, the effects of accessory cells, particularly natural killer (NK) cells, on human CB HSC (CBSC) functions were assessed in vitro and in vivo. CBSC cultured with autologous CB NK cells showed higher levels of CXCR4 expression, a higher migration index and a higher number of colony forming units (CFU) after short-term and long-term cultures. We found that CBSC secreted CXCL9 following interaction with CB NK cells. In addition, recombinant CXCL9 increased CBSC clonogenicity, recapitulating the effect observed of CB NK cells on CBSC. Moreover, the co-infusion of CBSC with CB NK cells led to a higher level of CBSC engraftment in NSG mouse model. The results presented in this work offer the basis for an alternative approach to enhance HSC engraftment that could improve the outcome of CBT.
PB Public Library of Science
YR 2015
FD 2015
LK http://hdl.handle.net/10668/2248
UL http://hdl.handle.net/10668/2248
LA en
NO Escobedo-Cousin M, Jackson N, Laza-Briviesca R, Ariza-McNaughton L, Luevano M, Derniame S, et al. Natural Killer Cells Improve Hematopoietic Stem Cell Engraftment by Increasing Stem Cell Clonogenicity In Vitro and in a Humanized Mouse Model. PLoS ONE; 10(10):e0138623
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 18, 2025