RT Journal Article
T1 Genetic Polymorphisms of Superoxide Dismutase Locus of Pneumocystis jirovecii in Spanish Population.
A1 Morilla, Ruben
A1 Gonzalez-Magaña, Amaia
A1 Friaza, Vicente
A1 de-Armas, Yaxsier
A1 Medrano, Francisco J
A1 Calderon, Enrique J
A1 de-la-Horra, Carmen
K1 Pneumocystis
K1 Spain
K1 colonization
K1 molecular epidemiology
K1 superoxide dismutase
AB Objective:Pneumocystis pneumonia remains a major opportunistic infection in immunocompromised patients worldwide. Colonization with Pneumocystis jirovecii has recently gained attention as an important issue for understanding the complete cycle of human Pneumocystis infection. P. jirovecii Superoxide Dismutase (SOD) gene could be a molecular target with high clinical relevance, but the epidemiological information about SOD genotypes distribution is scarce. The aim of this work was to provide information about the prevalence of genotypes of Pneumocystis SOD among Spanish patients and to describe possible differences between colonized and Pneumocystis pneumonia patients. Methods: we developed a cross-sectional study analyzing broncho-alveolar lavage fluid samples from 30 Pneumocystis pneumonia patients, 30 colonized patients, and 20 controls using a nested PCR protocol designed to amplify the sodA gene of P. jirovecii. The diagnostic yield of SOD Nested PCR was evaluated against the routine practice of mtLSUrRNA Nested PCR, which is considered the gold standard. Results: SOD locus was amplified in 90% of Pneumocystis pneumonia patients, 10% of colonized patients, and none of controls. Genotype SOD1 was observed in 11 cases (52.4%) and genotype SOD2 in 10 cases (47.6%). Genotype SOD2 was observed only in Pneumocystis pneumonia patients while the genotype SOD1 was observed in both colonized and Pneumocystis pneumonia patients. Conclusions: This study provides epidemiological information about SOD genotypes distribution in Spain, showing a low genetic diversity and a predominant presence of genotype SOD1 in colonized patients. SOP Nested PCR was more sensitive and accurate assay in Pneumocystis pneumonia patients than in colonized individuals.
PB Frontiers Research Foundation
SN 2296-2565
YR 2019
FD 2019-10-15
LK http://hdl.handle.net/10668/14626
UL http://hdl.handle.net/10668/14626
LA en
NO Morilla R, González-Magaña A, Friaza V, de Armas Y, Medrano FJ, Calderón EJ, et al. Genetic Polymorphisms of Superoxide Dismutase Locus of Pneumocystis jirovecii in Spanish Population. Front Public Health. 2019 Oct 15;7:292.
DS RISalud
RD Apr 9, 2025