RT Journal Article
T1 Screening a Protein Array with Synthetic Biotinylated Inorganic Polyphosphate To Define the Human PolyP-ome.
A1 Azevedo, Cristina
A1 Singh, Jyoti
A1 Steck, Nicole
A1 Hofer, Alexandre
A1 Ruiz, Felix A
A1 Singh, Tanya
A1 Jessen, Henning J
A1 Saiardi, Adolfo
K1 Protein Domains
K1 Protein Processing, Post-Translational
K1 Proteome
K1 Proteomics
AB Phenotypes are established by tight regulation on protein functions. This regulation can be mediated allosterically, through protein binding, and covalently, through post-translational modification (PTM). The integration of an ever-increasing number of PTMs into regulatory networks enables and defines the proteome complexity. Protein PTMs can occur enzymatically and nonenzymatically. Polyphosphorylation, which is a recently discovered PTM that belongs to the latter category, is the covalent attachment of the linear ortho-phosphate polymer called inorganic polyphosphate (polyP) to lysine residues. PolyP, which is ubiquitously present in nature, is also known to allosterically control protein function. To date, lack of reagents has prevented the systematic analysis of proteins covalently and/or allosterically associated with polyP. Here, we report on the chemical synthesis of biotin-modified monodisperse short-chain polyP (bio-polyP8-bio) and its subsequent use to screen a human proteome array to identify proteins that associate with polyP, thereby starting to define the human polyP-ome.
PB American Chemical Society
YR 2018
FD 2018-06-20
LK http://hdl.handle.net/10668/12621
UL http://hdl.handle.net/10668/12621
LA en
NO Azevedo C, Singh J, Steck N, Hofer A, Ruiz FA, Singh T, et al. Screening a Protein Array with Synthetic Biotinylated Inorganic Polyphosphate To Define the Human PolyP-ome. ACS Chem Biol. 2018 Aug 17;13(8):1958-1963
NO This work was supported by the MedicalResearch Council (MRC) core support to the MRC/UCLLaboratory for Molecular Cell Biology University Unit,MC_U12266B to T.S. (Bioinformatics Image Core) andMC_UU_1201814 to C.A. and A.S. and an HFSP programgrant (RPG0025/2016) to J.S., N.S., and H.J. F.R. was therecipient of a scholarship grant from the Government of Spain(“Salvador de Madariaga” program MECD PRX17/00473).
DS RISalud
RD Apr 12, 2025