RT Journal Article
T1 CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells.
A1 Alvarez, Belen
A1 Revilla, Concepcion
A1 Moreno, Sara
A1 Jimenez-Marin, Angeles
A1 Ramos, Elena
A1 Martinez de la Riva, Paloma
A1 Poderoso, Teresa
A1 Garrido, Juan J
A1 Ezquerra, Angel
A1 Dominguez, Javier
K1 CCR7
K1 CD4 T cells
K1 CD9
K1 IFN-γ
K1 Memory
K1 Swine
AB In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4+ T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4+ T cell development. CD4+ 2E3+ CD8α- CD9- cells are the least differentiated population of naïve cells, whereas the CD4+ 2E3- CD8α+CD9+ and CD4+ 2E3- CD8α+ CD9- cells display phenotypic features of central and effector memory T helper cells, respectively. The latter subsets were able to produce IFN-γ after polyclonal activation with PMA/Ionomycin; however, in vitro virus-specific IFN-γ production of PBMCs collected at 38-44 days after pseudorabies virus vaccination was dominated by cells with a CD9+ phenotype. Therefore, CD9 appears to be a useful marker to investigate CD4+ T cell heterogeneity in swine.
PB Elsevier
YR 2022
FD 2022-05-02
LK http://hdl.handle.net/10668/22152
UL http://hdl.handle.net/10668/22152
LA en
NO Álvarez B, Revilla C, Moreno S, Jiménez-Marín Á, Ramos E, Martínez de la Riva P, et al. CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells. Dev Comp Immunol. 2022 Aug;133:104431
DS RISalud
RD Apr 7, 2025