%0 Journal Article
%A Alvarez, Belen
%A Revilla, Concepcion
%A Moreno, Sara
%A Jimenez-Marin, Angeles
%A Ramos, Elena
%A Martinez de la Riva, Paloma
%A Poderoso, Teresa
%A Garrido, Juan J
%A Ezquerra, Angel
%A Dominguez, Javier
%T CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells.
%D 2022
%U http://hdl.handle.net/10668/22152
%X In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4+ T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4+ T cell development. CD4+ 2E3+ CD8α- CD9- cells are the least differentiated population of naïve cells, whereas the CD4+ 2E3- CD8α+CD9+ and CD4+ 2E3- CD8α+ CD9- cells display phenotypic features of central and effector memory T helper cells, respectively. The latter subsets were able to produce IFN-γ after polyclonal activation with PMA/Ionomycin; however, in vitro virus-specific IFN-γ production of PBMCs collected at 38-44 days after pseudorabies virus vaccination was dominated by cells with a CD9+ phenotype. Therefore, CD9 appears to be a useful marker to investigate CD4+ T cell heterogeneity in swine.
%K CCR7
%K CD4 T cells
%K CD9
%K IFN-γ
%K Memory
%K Swine
%~