RT Journal Article
T1 Detergent-Based Protocols on Decellularization of Corneas With Sclerocorneal Limbus. Evaluation of Regional Differences.
A1 González-Andrades, Miguel
A1 Carriel, Victor
A1 Rivera-Izquierdo, Mario
A1 Garzón, Ingrid
A1 González-Andrades, Elena
A1 Medialdea, Santiago
A1 Alaminos, Miguel
A1 Campos, Antonio
K1 Cornea regions
K1 Decellularization protocols
K1 Tissue engineering
K1 Sclerocorneal limbus
K1 Extracellular matrix
K1 Animales
K1 Compuestos de benzalconio
K1 Lámina limitante anterior
K1 Colágeno
K1 Córnea
K1 Trasplante de córnea
K1 Lámina limitante posterior
K1 Detergentes
K1 Limbo de la córnea
K1 Octoxinol
K1 Proteoglicanos
K1 Sodio
K1 Dodecil sulfato de sodio
K1 Porcinos
AB PURPOSEIn this work, we decellularized whole porcine corneas including the sclerocorneal limbus (SCL) and we evaluated regional differences in order to identify an efficient method to decellularize whole corneas for future clinical use.METHODSWe analyzed the efficiency of four decellularization protocols based on benzalkonium chloride (BAK), Igepal, sodium dodecyl sulfate (SDS), and Triton X-100 detergents on whole porcine corneas.RESULTSResults showed that the decellularization efficiency of most protocols was low, with the specific protocol resulting in more efficient levels of decellularization being 0.1% SDS for 48 hours, especially in the medium and posterior cornea regions. A significant correlation was found between the decellularization efficiency and the concentration of agent used (P = 0.0174; r = 0.1540), but not for the incubation time (P > 0.05). The analysis of cornea components preservation demonstrated that all protocols were able to preserve the integrity of the Bowman's layer and Descemet's membrane. Although the collagen structure was partially altered, the global decellularization groups showing highest preservation of the ECM collagen contents and orientation were Igepal and SDS, with differences among the three regions of the cornea. All global groups showed high levels of proteoglycan and glycoprotein preservation after decellularization, with the best results were found in the SDS group followed by the Igepal group.CONCLUSIONSThese results suggest that very powerful protocols are necessary for whole-cornea decellularization. For the generation of lamelar corneas for clinical use, decellularization regional differences should be taken into account.TRANSLATIONAL RELEVANCEDecellularized whole corneas may be potential therapeutic agents for lamelar keratoplasty.
PB Association for Research in Vision and Ophthalmology (ARVO)
YR 2015
FD 2015-04-10
LK http://hdl.handle.net/10668/2308
UL http://hdl.handle.net/10668/2308
LA en
NO González-Andrades M, Carriel V, Rivera-Izquierdo M, Garzón I, González-Andrades E, Medialdea S, et al. Effects of Detergent-Based Protocols on Decellularization of Corneas With Sclerocorneal Limbus. Evaluation of Regional Differences. Transl Vis Sci Technol. 2015                         ; 4(2):13
NO JOURNAL ARTICLE;
DS RISalud
RD Apr 18, 2025