RT Journal Article
T1 Adaptive Response of Saccharomyces Hosts to Totiviridae L-A dsRNA Viruses Is Achieved through Intrinsically Balanced Action of Targeted Transcription Factors.
A1 Ravoitytė, Bazilė
A1 Lukša, Juliana
A1 Wellinger, Ralf Erik
A1 Serva, Saulius
A1 Servienė, Elena
K1 Saccharomyces cerevisiae
K1 Saccharomyces paradoxus
K1 Totiviridae
K1 dsRNA
K1 transcription
K1 yeast
AB Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host's gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.
YR 2022
FD 2022-04-09
LK http://hdl.handle.net/10668/21368
UL http://hdl.handle.net/10668/21368
LA en
DS RISalud
RD Apr 10, 2025