RT Journal Article
T1 Xrn1 influence on gene transcription results from the combination of general effects on elongating RNA pol II and gene-specific chromatin configuration.
A1 Begley, Victoria
A1 Jordán-Pla, Antonio
A1 Peñate, Xenia
A1 Garrido-Godino, Ana I
A1 Challal, Drice
A1 Cuevas-Bermúdez, Abel
A1 Mitjavila, Adrià
A1 Barucco, Mara
A1 Gutiérrez, Gabriel
A1 Singh, Abhyudai
A1 Alepuz, Paula
A1 Navarro, Francisco
A1 Libri, Domenico
A1 Pérez-Ortín, José E
A1 Chávez, Sebastián
K1 3ʹ pre-mRNA processing
K1 Transcription elongation
K1 Xrn1
K1 mRNA buffering
K1 mRNA decay
K1 mRNA decay/gene transcription crosstalk
AB mRNA homoeostasis is favoured by crosstalk between transcription and degradation machineries. Both the Ccr4-Not and the Xrn1-decaysome complexes have been described to influence transcription. While Ccr4-Not has been shown to directly stimulate transcription elongation, the information available on how Xrn1 influences transcription is scarce and contradictory. In this study we have addressed this issue by mapping RNA polymerase II (RNA pol II) at high resolution, using CRAC and BioGRO-seq techniques in Saccharomyces cerevisiae. We found significant effects of Xrn1 perturbation on RNA pol II profiles across the genome. RNA pol II profiles at 5' exhibited significant alterations that were compatible with decreased elongation rates in the absence of Xrn1. Nucleosome mapping detected altered chromatin configuration in the gene bodies. We also detected accumulation of RNA pol II shortly upstream of polyadenylation sites by CRAC, although not by BioGRO-seq, suggesting higher frequency of backtracking before pre-mRNA cleavage. This phenomenon was particularly linked to genes with poorly positioned nucleosomes at this position. Accumulation of RNA pol II at 3' was also detected in other mRNA decay mutants. According to these and other pieces of evidence, Xrn1 seems to influence transcription elongation at least in two ways: by directly favouring elongation rates and by a more general mechanism that connects mRNA decay to late elongation.
YR 2020
FD 2020-12-01
LK http://hdl.handle.net/10668/16530
UL http://hdl.handle.net/10668/16530
LA en
DS RISalud
RD Apr 6, 2025