%0 Journal Article
%A Valhondo, Isabel
%A Hassouneh, Fakhri
%A Lopez-Sejas, Nelson
%A Pera, Alejandra
%A Sanchez-Correa, Beatriz
%A Guerrero, Beatriz
%A Bergua, Juan M
%A Arcos, Maria Jose
%A Bañas, Helena
%A Casas-Avilés, Ignacio
%A Sanchez-Garcia, Joaquin
%A Serrano, Josefina
%A Martin, Carmen
%A Duran, Esther
%A Alonso, Corona
%A Solana, Rafael
%A Tarazona, Raquel
%T Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia.
%D 2020
%@ 2072-6694
%U https://hdl.handle.net/10668/25804
%X Background: Acute myeloid leukemia (AML) remains a major clinical challenge due to poor overall survival, which is even more dramatic in elderly patients. TIGIT, an inhibitory receptor that interacts with CD155 and CD112 molecules, is considered as a checkpoint in T and NK cell activation. This receptor shares ligands with the co-stimulatory receptor DNAM-1 and with TACTILE. The aim of this work was to analyze the expression of DNAM-1, TIGIT and TACTILE in NK cells and T cell subsets in AML patients. Methods: We have studied 36 patients at the time of diagnosis of AML and 20 healthy volunteers. The expression of DNAM-1, TIGIT and TACTILE in NK cells and T cells, according to the expression of CD3 and CD56, was performed by flow cytometry. Results: NK cells, CD56- T cells and CD56+ T (NKT-like) cells from AML patients presented a reduced expression of DNAM-1 compared with healthy volunteers. An increased expression of TIGIT was observed in mainstream CD56- T cells. No differences were observed in the expression of TACTILE. Simplified presentation of incredibly complex evaluations (SPICE) analysis of the co-expression of DNAM-1, TIGIT and TACTILE showed an increase in NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE. Low percentages of DNAM-1-TIGIT+TACTILE+ NK cells and DNAM-1- TIGIT+TACTILE+ CD56- T cells were associated with a better survival of AML patients. Conclusions: The expression of DNAM-1 is reduced in NK cells and in CD4+ and CD8+ T cells from AML patients compared with those from healthy volunteers. An increased percentage of NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE is associated with patient survival, supporting the role of TIGIT as a novel candidate for checkpoint blockade.
%K DNAM-1
%K NK cells
%K NKT-like cells
%K T cells
%K TACTILE
%K TIGIT
%K acute myeloid leukemia
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