RT Journal Article
T1 The Inhibitory Role of miR-486-5p on CSC Phenotype Has Diagnostic and Prognostic Potential in Colorectal Cancer.
A1 Pisano, Andrea
A1 Griñan-Lison, Carmen
A1 Farace, Cristiano
A1 Fiorito, Giovanni
A1 Fenu, Grazia
A1 Jiménez, Gema
A1 Scognamillo, Fabrizio
A1 Peña-Martin, Jesùs
A1 Naccarati, Alessio
A1 Pröll, Johannes
A1 Atzmüller, Sabine
A1 Pardini, Barbara
A1 Attene, Federico
A1 Ibba, Gabriele
A1 Solinas, Maria Giuliana
A1 Bernhard, David
A1 Marchal, Juan Antonio
A1 Madeddu, Roberto
K1 cancer stem cells
K1 colorectal cancer
K1 metastasis
K1 miR-486-5p
K1 serum and stool biomarkers
AB Colorectal cancer (CRC) is the third most frequent cancer worldwide and the second cause of cancer deaths. Increasing evidences supports the idea that the poor prognosis of patients is related to the presence of cancer stem cells (CSCs), a cell population able to drive cancer recurrence and metastasis. The deregulation of microRNAs (miRNAs) plays a role in the formation of CSC. We investigated the role of hsa-miR-486-5p (miR-486-5p) in CRC, CSCs, and metastasis, in order to reach a better understanding of the biomolecular and epigenetic mechanisms mir-486-5p-related. The expression of miR-486-5p was investigated in three different matrices from CRC patients and controls and in CSCs obtained from the CRC cell lines HCT-116, HT-29, and T-84. In the human study, miR-486-5p was up-regulated in serum and stool of CRC patients in comparison with healthy controls but down-regulated in tumor tissue when compared with normal mucosa. miR-486-5p was also down-regulated in the sera of metastatic patients. In vitro, miR-486-5p was down-regulated in CSC models and it induced an inhibitory effect on stem factors and oncogenes in the main pathways of CSCs. Our results provide a step forward in understanding the role of mir-486-5p in CRC and CSC, and suggest that further studies are needed to investigate its diagnostic and prognostic power, possibly in combination with other biomarkers.
SN 2072-6694
YR 2020
FD 2020-11-19
LK https://hdl.handle.net/10668/28154
UL https://hdl.handle.net/10668/28154
LA en
DS RISalud
RD Apr 12, 2025