RT Journal Article
T1 LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model.
A1 Rodriguez, Maria J
A1 Aranda, Ana
A1 Fernandez, Tahia D
A1 Cubells-Baeza, Nuria
A1 Torres, Maria J
A1 Gomez, Francisca
A1 Palomares, Francisca
A1 Perkins, James R
A1 Rojo, Javier
A1 Diaz-Perales, Araceli
A1 Mayorga, Cristobalina
K1 Immunoglobulin E
K1 Immunoglobulin G
K1 Lipopolysaccharides
K1 Mice, Inbred BALB C
K1 Spleen
AB Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4+ T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies.
PB Nature Publishing Group
YR 2017
FD 2017-01-13
LK http://hdl.handle.net/10668/10771
UL http://hdl.handle.net/10668/10771
LA en
NO Rodriguez MJ, Aranda A, Fernandez TD, Cubells-Baeza N, Torres MJ, Gomez F, et al. LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model. Sci Rep. 2017 Jan 13;7:40449
NO The study was funded by the Institute of Health “Carlos III” of the Ministry of Economy and Competitiveness (grants cofounded by European Regional Development Fund (ERDF): PI12/02481 and PI15/00559, Red de Reacciones Adversas a Alergenos y Farmacos RD 07/0064, RD12/0013/0001, RD07/0064/0003 and RD12/0013/0016). Andalusian Regional Ministry of Economy and Knowledge (grants cofounded by European Regional Development Fund (ERDF): CTS-7433), Nicolas Monardes Program (C-0044-2012 SAS2013), Sara Borrell Program (Ref CD14/00242) and BIO201341403-R.
DS RISalud
RD Apr 19, 2025