RT Journal Article
T1 Optical Behavior of Human Skin Substitutes: Absorbance in the 200-400 nm UV Range
A1 Ruiz-Lopez, Javier
A1 Cardona, Juan C.
A1 Garzon, Ingrid
A1 Perez, Maria M.
A1 Alaminos, Miguel
A1 Chato-Astrain, Jesus
A1 Ionescu, Ana M.
K1 absorption
K1 UV radiation
K1 bioengineered skin
K1 fibrin-agarose biomaterial
K1 Ultraviolet-radiation
K1 Stem-cells
K1 Biomaterials
K1 Photobiology
K1 Protein
AB The most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmittance of the UGRSKIN bioartificial skin substitute generated with human skin cells combined with fibrin-agarose biomaterials were evaluated for: UV-C (200-280 nm), -B (280-315 nm), and -A (315-400 nm) spectral range after 7, 14, 21 and 28 days of ex vivo development. The epidermis of the bioartificial skin substitute was able to mature and differentiate in a time-dependent manner, expressing relevant molecules able to absorb most of the incoming UV radiation. Absorbance spectral behavior of the skin substitutes showed similar patterns to control native skin (VAF > 99.4%), with values 0.85-0.90 times lower than control values at 7 and 14- days and 1.05-1.10 times the control values at 21- and 28-days. UV absorbance increased, and UV transmission decreased with culture time, and comparable results to the control were found at 21 and 28 days. These findings support the use of samples corresponding to 21 or 28 days of development for clinical purposes due to their higher histological similarities with native skin, but also because of their absorbance of UV radiation.
PB Mdpi
YR 2022
FD 2022-07-01
LK http://hdl.handle.net/10668/20840
UL http://hdl.handle.net/10668/20840
LA en
DS RISalud
RD Apr 7, 2025