RT Journal Article
T1 Vitamin E protects against extraskeletal calcification in uremic rats fed high fat diets.
A1 Rios, Rafael
A1 Raya, Ana I
A1 Pineda, Carmen
A1 Rodriguez, Mariano
A1 Lopez, Ignacio
A1 Aguilera-Tejero, Escolastico
K1 High-fat diet
K1 Uremia
K1 Vascular calcification
K1 Vitamin E
AB High fat diets are implicated in the pathogenesis of metabolic syndrome, obesity and renal disease. Previous studies have revealed that high fat diets promote vascular calcification in uremic rats. Moreover, vitamin E has been shown to prevent uremic calcifications in genetically obese Zucker rats fed standard diet. The objective of this study was to investigate the influence of vitamin E supplementation on the development of extraskeletal calcifications in non-obese (wild type) uremic rats fed high fat diets. Wistar rats (n = 32) were preconditioned by feeding either a normal (NF) or high fat (HF) diet for 45 days and subsequently were subjected to 5/6 nephrectomy (Nx). Just before performing the first Nx step, a blood sample (Pre-Nx) was obtained. After Nx rats were switched to a diet with 0.9% phosphorus and supplemented with calcitriol. Also, after Nx, half of the rats from each group (NF and HF) were treated with vitamin E (VitE) in the diet (30,000 mg/kg) and the other half were maintained on basic VitE requirements (27 mg/kg). Thus, rats were allotted to four experimental groups: Nx-NF (n = 8), Nx-NF-VitE (n = 8), Nx-HF (n = 8) and Nx-HF-VitE (n = 8). At the time of sacrifice (day 66), blood and tissue samples were obtained. Feeding a HF diet for 45 days did not increase body weight but elicited hyperglycemia, hypertriglyceridemia, an increase in plasma fibroblast growth factor 23 and a reduction in plasma calcitriol concentrations. After Nx, rats fed HF diet showed substantial extraskeletal calcification with aortic calcium content that was higher than in rats fed NF diet. Supplementation with VitE significantly (p  Uremic rats fed HF diets developed more severe extraosseous calcifications than their normocaloric-fed counterparts and dietary VitE supplementation protected against uremic calcifications in rats fed HF diets. Thus, eating energy-rich foods should be discouraged in patients with renal disease and their deleterious effect may be ameliorated with adequate antioxidant supply.
PB BioMed Central
YR 2017
FD 2017-12-12
LK http://hdl.handle.net/10668/11956
UL http://hdl.handle.net/10668/11956
LA en
NO Rios R, Raya AI, Pineda C, Rodriguez M, Lopez I, Aguilera-Tejero E. Vitamin E protects against extraskeletal calcification in uremic rats fed high fat diets. BMC Nephrol. 2017 Dec 28;18(1):374
DS RISalud
RD Apr 6, 2025