RT Journal Article
T1 Crohn's Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat.
A1 Madeira, Ana
A1 Serena, Carolina
A1 Ejarque, Miriam
A1 Maymo-Masip, Elsa
A1 Millan, Monica
A1 Navarro-Ruiz, M Carmen
A1 Guzman-Ruiz, Rocio
A1 Malagon, Maria M
A1 Espin, Eloy
A1 Marti, Marc
A1 Menacho, Margarita
A1 Megia, Ana
A1 Vendrell, Joan
A1 Fernandez-Veledo, Sonia
K1 Crohn’s disease
K1 Adipose tissue
K1 Adipose-derived stem cells
K1 Mesothelium
AB Our understanding of the interplay between human adipose tissue and the immune system is limited. The mesothelium, an immunologically active structure, emerged as a source of visceral adipose tissue. After investigating the mesothelial properties of human visceral and subcutaneous adipose tissue and their progenitors, we explored whether the dysfunctional obese and Crohn's disease environments influence the mesothelial/mesenchymal properties of their adipocyte precursors, as well as their ability to mount an immune response. Using a tandem transcriptomic/proteomic approach, we evaluated the mesothelial and mesenchymal expression profiles in adipose tissue, both in subjects covering a wide range of body-mass indexes and in Crohn's disease patients. We also isolated adipose tissue precursors (adipose-derived stem cells, ASCs) to assess their mesothelial/mesenchymal properties, as well as their antigen-presenting features. Human visceral tissue presented a mesothelial phenotype not detected in the subcutaneous fat. Only ASCs from mesenteric adipose tissue, named creeping fat, had a significantly higher expression of the hallmark mesothelial genes mesothelin (MSLN) and Wilms' tumor suppressor gene 1 (WT1), supporting a mesothelial nature of these cells. Both lean and Crohn's disease visceral ASCs expressed equivalent surface percentages of the antigen-presenting molecules human leucocyte antigen-DR isotype (HLA-DR) and CD86. However, lean-derived ASCs were predominantly HLA-DR dim, whereas in Crohn's disease, the HLA-DR bright subpopulation was increased 3.2-fold. Importantly, the mesothelial-enriched Crohn's disease precursors activated CD4+ T-lymphocytes. Our study evidences a mesothelial signature in the creeping fat of Crohn's disease patients and its progenitor cells, the latter being able to present antigens and orchestrate an immune response.
PB MDPI
YR 2021
FD 2021-04-16
LK http://hdl.handle.net/10668/17713
UL http://hdl.handle.net/10668/17713
LA en
NO Madeira A, Serena C, Ejarque M, Maymó-Masip E, Millan M, Navarro-Ruiz MC, et al. Crohn's Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat. Int J Mol Sci. 2021 Apr 20;22(8):4292
DS RISalud
RD Apr 19, 2025