RT Journal Article
T1 Exploring Honeybee Abdominal Anatomy through Micro-CT and Novel Multi-Staining Approaches.
A1 De Paula, Jessica Carreira
A1 Doello, Kevin
A1 Mesas, Cristina
A1 Kapravelou, Garyfalia
A1 Cornet-Gómez, Alberto
A1 Orantes, Francisco José
A1 Martínez, Rosario
A1 Linares, Fátima
A1 Prados, Jose Carlos
A1 Porres, Jesus María
A1 Osuna, Antonio
A1 de Pablos, Luis Miguel
K1 Micro-CT
K1 abdomen
K1 bee
K1 midgut
K1 stain
AB Continuous improvements in morphological and histochemical analyses of Apis mellifera could improve our understanding of the anatomy and physiology of these insects at both the cellular and tissue level. In this work, two different approaches have been performed to add new data on the abdomen of worker bees: (i) Micro-computed tomography (Micro-CT), which allows the identification of small-scale structures (micrometers) with adequate/optimal resolution and avoids sample damage and, (ii) histochemical multi-staining with Periodic Acid-Schiff-Alcian blue, Lactophenol-Saphranin O and pentachrome staining to precisely characterize the histological structures of the midgut and hindgut. Micro-CT allowed high-resolution imaging of anatomical structures of the honeybee abdomen with particular emphasis on the proventriculus and pyloric valves, as well as the connection of the sting apparatus with the terminal abdominal ganglia. Furthermore, the histochemical analyses have allowed for the first-time description of ventricular telocytes in honeybees, a cell type located underneath the midgut epithelium characterized by thin and long cytoplasmic projections called telopodes. Overall, the analysis of these images could help the detailed anatomical description of the cryptic structures of honeybees and also the characterization of changes due to abiotic or biotic stress conditions.
SN 2075-4450
YR 2022
FD 2022-06-18
LK http://hdl.handle.net/10668/21218
UL http://hdl.handle.net/10668/21218
LA en
DS RISalud
RD Apr 11, 2025