RT Journal Article
T1 Modified sequential multiplex PCR for determining capsular serotypes of invasive pneumococci recovered from Seville.
A1 Iraurgui, P
A1 Torres, María José
A1 Vazquez, I
A1 García-Cabrera, Emilio
A1 Obando, Ignacio
A1 Garnacho-Montero, José
A1 Aznar, Javier
K1 Capsular polysaccharide
K1 Invasive infection
K1 Multiplex PCR
K1 Serotype
K1 Streptococcus pneumoniae
AB The heptavalent pneumococcal vaccine's introduction resulted in a decline in invasive disease caused by Streptococcus pneumoniae, but was accompanied by an increase in non-vaccine serotypes. We evaluated a modified scheme of the sequential multiplex PCRs adapted to the prevalence of serotypes in Seville (Spain) for determining capsular serotypes of S. pneumoniae invasive clinical isolates. In adults, the modified scheme allowed us to type 73% with the first three reactions, and 92% with two additional PCRs. In paediatric patients, it allowed us to type 73.5% with the first three reactions, and 90% with the two additional PCRs. The multiplex PCR approach was successfully adapted to target the serotypes most prevalent in Seville.
PB Elsevier
SN 1469-0691
YR 2010
FD 2010-09
LK https://hdl.handle.net/10668/28449
UL https://hdl.handle.net/10668/28449
LA en
NO Iraurgui P, Torres MJ, Gandia A, Vazquez I, Cabrera EG, Obando I, et al. Modified sequential multiplex PCR for determining capsular serotypes of invasive pneumococci recovered from Seville. Clin Microbiol Infect. 2010 Sep;16(9):1504-7.
DS RISalud
RD Apr 8, 2025