RT Journal Article
T1 Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples.
A1 Pla, Luis
A1 Aviñó, Anna
A1 Eritja, Ramón
A1 Ruiz-Gaitán, Alba
A1 Pemán, Javier
A1 Friaza, Vicente
A1 Calderón, Enrique J
A1 Aznar, Elena
A1 Martínez-Máñez, Ramón
A1 Santiago-Felipe, Sara
K1 Pneumocystis jirovecii
K1 biosensor
K1 molecular gates
K1 nanoporous anodic alumina
K1 oligonucleotides
AB Pneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus Pneumocystis jirovecii. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of P. jirovecii is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of P. jirovecii genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with P. jirovecii DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from P. jirovecii in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia.
YR 2020
FD 2020-11-17
LK https://hdl.handle.net/10668/27505
UL https://hdl.handle.net/10668/27505
LA en
DS RISalud
RD Apr 12, 2025