RT Journal Article
T1 Improvement in detecting cytomegalovirus drug resistance mutations in solid organ transplant recipients with suspected resistance using next generation sequencing.
A1 Lopez-Aladid, Ruben
A1 Guiu, Alba
A1 Mosquera, Maria Mar
A1 Lopez-Medrano, Francisco
A1 Cofan, Frederic
A1 Linares, Laura
A1 Torre-Cisneros, Julian
A1 Vidal, Elisa
A1 Moreno, Asuncion
A1 Aguado, Jose Maria
A1 Cordero, Elisa
A1 Martin-Gandul, Cecilia
A1 Carratala, Jordi
A1 Sabe, Nuria
A1 Niubo, Jordi
A1 Cervera, Carlos
A1 Capon, Alicia
A1 Cervilla, Anna
A1 Santos, Marta
A1 Bodro, Marta
A1 Muñoz, Patricia
A1 Fariñas, Maria Carmen
A1 Antón, Andres
A1 Aranzamendi, Maitane
A1 Montejo, Miguel
A1 Perez-Romero, Pilar
A1 Len, Oscar
A1 Marcos, Maria Angeles
K1 Cytomegalovirus
K1 Drug Resistance, Viral
K1 Female
K1 Genes, Viral
K1 Farmacorresistencia viral
AB The aim of this study was to identify CMV drug resistance mutations (DRM) in solid organ transplant (SOT) recipients with suspected resistance comparing next-generation sequencing (NGS) with Sanger sequencing and assessing risk factors and the clinical impact of resistance. Using Sanger sequencing as the reference method, we prospectively assessed the ability of NGS to detect CMV DRM in the UL97 and UL54 genes in a nationwide observational study from September 2013 to August 2016. Among 44 patients recruited, 14 DRM were detected by Sanger in 12 patients (27%) and 20 DRM were detected by NGS, in 16 (36%). NGS confirmed all the DRM detected by Sanger. The additional six mutations detected by NGS were present in  NGS showed a higher yield than Sanger sequencing for detecting CMV resistance mutations in SOT recipients. The presence of DRM detected by NGS was independently associated with longer antiviral treatment.
PB Public Library of Science
YR 2019
FD 2019-05-07
LK http://hdl.handle.net/10668/14252
UL http://hdl.handle.net/10668/14252
LA en
NO López-Aladid R, Guiu A, Mosquera MM, López-Medrano F, Cofán F, Linares L, et al. Improvement in detecting cytomegalovirus drug resistance mutations in solid organ transplant recipients with suspected resistance using next generation sequencing. PLoS One. 2019 Jul 18;14(7):e0219701
DS RISalud
RD Apr 7, 2025