RT Journal Article
T1 Harmful DNA:RNA hybrids are formed in cis and in a Rad51-independent manner
A1 Lafuente-Barquero, Juan
A1 García-Rubio, Maria Luisa
A1 Martin-Alonso, Marta San
A1 Gómez-González, Belén
A1 Aguilera, Andrés
K1 RNA
K1 DNA
K1 Rad51
K1 DNA Damage
K1 Genome
K1 ARN
K1 ADN
K1 Rad51 recombinasa
K1 Daño del ADN
K1 Genoma
AB DNA:RNA hybrids constitute a well-known source of recombinogenic DNA damage. The current literature is in agreement with DNA:RNA hybrids being produced co-transcriptionally by the invasion of the nascent RNA molecule produced in cis with its DNA template. However, it has also been suggested that recombinogenic DNA:RNA hybrids could be facilitated by the invasion of RNA molecules produced in trans in a Rad51-mediated reaction. Here, we tested the possibility that such DNA:RNA hybrids constitute a source of recombinogenic DNA damage taking advantage of Rad51-independent single-strand annealing (SSA) assays in the yeast Saccharomyces cerevisiae. For this, we used new constructs designed to induce expression of mRNA transcripts in trans with respect to the SSA system. We show that unscheduled and recombinogenic DNA:RNA hybrids that trigger the SSA event are formed in cis during transcription and in a Rad51-independent manner. We found no evidence that such hybrids form in trans and in a Rad51-dependent manner.
PB eLife Sciences Publications Ltd.
YR 2020
FD 2020-08-04
LK http://hdl.handle.net/10668/3851
UL http://hdl.handle.net/10668/3851
LA en
NO Lafuente-Barquero J, García-Rubio ML, Martin-Alonso MS, Gómez-González B, Aguilera A. Harmful DNA:RNA hybrids are formed in cis and in a Rad51-independent manner. Elife. 2020 Aug 4;9:e56674
DS RISalud
RD Apr 9, 2025