RT Journal Article
T1 Human mesenchymal stem cells enhance the systemic effects of radiotherapy.
A1 de Araújo Farias, Virgínea
A1 O'Valle, Francisco
A1 Lerma, Borja Alonso
A1 Ruiz de Almodóvar, Carmen
A1 López-Peñalver, Jesús J
A1 Nieto, Ana
A1 Santos, Ana
A1 Fernández, Beatriz Irene
A1 Guerra-Librero, Ana
A1 Ruiz-Ruiz, María Carmen
A1 Guirado, Damián
A1 Schmidt, Thomas
A1 Oliver, Francisco Javier
A1 Ruiz de Almodóvar, José Mariano
K1 Radiotherapy
K1 Bystander effect
K1 Mesenchymal cells
K1 Cancer
K1 Radiosensitizers
K1 Western Blotting
K1 Proliferación de la célula
K1 Radioisótopos de cesio
K1 Humanos
K1 Técnicas para inmunoenzimas
K1 Trasplante de células madre mesenquimatosas
K1 Células del estroma mesenquimatoso
K1 Ratones
K1 Ratones SCID
K1 Reacción en cadena en tiempo real de la polimerasa
K1 Reacción en cadena de la polimerasa de transcriptasa inversa
K1 Células tumorales cultivadas
K1 Ensayos antitumor por modelo de xenoinjerto
K1 Animales
AB The outcome of radiotherapy treatment might be further improved by a better understanding of individual variations in tumor radiosensitivity and normal tissue reactions, including the bystander effect. For many tumors, however, a definitive cure cannot be achieved, despite the availablity of more and more effective cancer treatments. Therefore, any improvement in the efficacy of radiotherapy will undoubtedly benefit a significant number of patients. Many experimental studies measure a bystander component of tumor cell death after radiotherapy, which highlights the importance of confirming these observations in a preclinical situation. Mesenchymal stem cells (MSCs) have been investigated for use in the treatment of cancers as they are able to both preferentially home onto tumors and become incorporated into their stroma. This process increases after radiation therapy. In our study we show that in vitro MSCs, when activated with a low dose of radiation, are a source of anti-tumor cytokines that decrease the proliferative activity of tumor cells, producing a potent cytotoxic synergistic effect on tumor cells. In vivo administration of unirradiated mesenchymal cells together with radiation leads to an increased efficacy of radiotherapy, thus leading to an enhancement of short and long range bystander effects on primary-irradiated tumors and distant-non-irradiated tumors. Our experiments indicate an increased cell loss rate and the decrease in the tumor cell proliferation activity as the major mechanisms underlying the delayed tumor growth and are a strong indicator of the synergistic effect between RT and MSC when they are applied together for tumor treatment in this model.
PB Impact Journals
YR 2015
FD 2015-10-13
LK http://hdl.handle.net/10668/2566
UL http://hdl.handle.net/10668/2566
LA en
NO de Araújo Farias V, O'Valle F, Lerma BA, Ruiz de Almodóvar C, López-Peñalver JJ, Nieto A, et al. Human mesenchymal stem cells enhance the systemic effects of radiotherapy. Oncotarget. 2015                         ; 6(31):31164-80
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 10, 2025