RT Journal Article T1 A Functional Link between AMPK and Orexin Mediates the Effect of BMP8B on Energy Balance. A1 Martins, Luis A1 Seoane-Collazo, Patricia A1 Contreras, Cristina A1 Gonzalez-Garcia, Ismael A1 Martinez-Sanchez, Noelia A1 Gonzalez, Francisco A1 Zalvide, Juan A1 Gallego, Rosalia A1 Dieguez, Carlos A1 Nogueiras, Ruben A1 Tena-Sempere, Manuel A1 Lopez, Miguel K1 Orexin Receptors K1 Orexins K1 Rats, Sprague-Dawley K1 Sex Factors K1 Signal Transduction K1 Thermogenesis K1 Ventromedial Hypothalamic Nucleus K1 Vesicular Glutamate Transport Protein 2 AB AMP-activated protein kinase (AMPK) in the ventromedial nucleus of the hypothalamus (VMH) and orexin (OX) in the lateral hypothalamic area (LHA) modulate brown adipose tissue (BAT) thermogenesis. However, whether these two molecular mechanisms act jointly or independently is unclear. Here, we show that the thermogenic effect of bone morphogenetic protein 8B (BMP8B) is mediated by the inhibition of AMPK in the VMH and the subsequent increase in OX signaling via the OX receptor 1 (OX1R). Accordingly, the thermogenic effect of BMP8B is totally absent in ox-null mice. BMP8B also induces browning of white adipose tissue (WAT), its thermogenic effect is sexually dimorphic (only observed in females), and its impact on OX expression and thermogenesis is abolished by the knockdown of glutamate vesicular transporter 2 (VGLUT2), implicating glutamatergic signaling. Overall, our data uncover a central network controlling energy homeostasis that may be of considerable relevance for obesity and metabolic disorders. PB Cell Press YR 2016 FD 2016-07-15 LK http://hdl.handle.net/10668/10359 UL http://hdl.handle.net/10668/10359 LA en NO Martins L, Seoane-Collazo P, Contreras C, González-García I, Martínez-Sánchez N, González F, et al. A Functional Link between AMPK and Orexin Mediates the Effect of BMP8B on Energy Balance. Cell Rep. 2016 Aug 23;16(8):2231-2242 DS RISalud RD Apr 12, 2025