RT Journal Article
T1 Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts.
A1 Melguizo-Rodríguez, Lucia
A1 Costela-Ruiz, Víctor J
A1 Manzano-Moreno, Francisco J
A1 Illescas-Montes, Rebeca
A1 Ramos-Torrecillas, Javier
A1 García-Martínez, Olga
A1 Ruiz, Concepción
K1 Bone tissue
K1 Differentiation
K1 Gene expression
K1 NSAIDs
K1 Osteoblasts
AB Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used in clinical practice, which can have adverse effects on the osteoblast. The objective of this study was to determine the effect of NSAIDs on the osteoblast by analyzing the gene expression of different markers related to osteoblast maturation and function when treated in vitro with different NSAIDs. Three human osteoblast lines from bone samples of three healthy volunteers were treated with 10 µM acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen, and piroxicam. The gene expression of different markers (run related transcription factor 2 [RUNX-2], type 1 collagen [COL-I], osterix [OSX], osteocalcin [OSC], bone morphogenetic protein 2 [BMP-2] and 7 [BMP-7], transforming growth factor β1 [TGF-β1], and TGFβ receptors [TGFβR1, TGFβR2; TGFBR3]) were analyzed by real-time PCR at 24 h of treatment. Expression of RUNX-2, COL-I, OSX, was reduced by treatment with all studied NSAIDs, OSC expression was reduced by all NSAIDs except for ketoprofen, naproxen, or piroxicam. Expression of BMP-7 was reduced by all NSAIDs; BMP-2 was reduced by all except for naproxen. In general, NSAID treatment increased the expression of TGF-β1, but not of its receptors (TGFβ-R1, TGFβ-R2, andTFGβ-R3), which was either unchanged or reduced by the treatment. These data confirm that NSAIDs can affect osteoblast physiology, suggesting their possible impact on bone.
SN 2167-8359
YR 2018
FD 2018-08-14
LK http://hdl.handle.net/10668/12859
UL http://hdl.handle.net/10668/12859
LA en
DS RISalud
RD Apr 9, 2025