RT Journal Article
T1 Glucose-dependent partitioning of arginine to the urea cycle protects β-cells from inflammation.
A1 Fu, Accalia
A1 Alvarez-Perez, Juan Carlos
A1 Avizonis, Daina
A1 Kin, Tatsuya
A1 Ficarro, Scott B
A1 Choi, Dong Wook
A1 Karakose, Esra
A1 Badur, Mehmet G
A1 Evans, Lindsay
A1 Rosselot, Carolina
A1 Bridon, Gaelle
A1 Bird, Gregory H
A1 Seo, Hyuk-Soo
A1 Dhe-Paganon, Sirano
A1 Kamphorst, Jurre J
A1 Stewart, Andrew F
A1 James Shapiro, A M
A1 Marto, Jarrod A
A1 Walensky, Loren D
A1 Jones, Russell G
A1 Garcia-Ocana, Adolfo
A1 Danial, Nika N
AB Chronic inflammation is linked to diverse disease processes, but the intrinsic mechanisms that determine cellular sensitivity to inflammation are incompletely understood. Here, we show the contribution of glucose metabolism to inflammation-induced changes in the survival of pancreatic islet β-cells. Using metabolomic, biochemical and functional analyses, we investigate the protective versus non-protective effects of glucose in the presence of pro-inflammatory cytokines. When protective, glucose metabolism augments anaplerotic input into the TCA cycle via pyruvate carboxylase (PC) activity, leading to increased aspartate levels. This metabolic mechanism supports the argininosuccinate shunt, which fuels ureagenesis from arginine and conversely diminishes arginine utilization for production of nitric oxide (NO), a chief mediator of inflammatory cytotoxicity. Activation of the PC-urea cycle axis is sufficient to suppress NO synthesis and shield cells from death in the context of inflammation and other stress paradigms. Overall, these studies uncover a previously unappreciated link between glucose metabolism and arginine-utilizing pathways via PC-directed ureagenesis as a protective mechanism.
YR 2020
FD 2020-05-11
LK https://hdl.handle.net/10668/26613
UL https://hdl.handle.net/10668/26613
LA en
DS RISalud
RD Apr 11, 2025