RT Journal Article
T1 Characterization of an AP endonuclease from sugarcane - ScARP1.
A1 Cabral Medeiros, Nathalia Maira
A1 Cordoba-Cañero, Dolores
A1 Garcia-Gil, Casimiro Barbado
A1 Ariza, Rafael R
A1 Roldan-Arjona, Teresa
A1 Scortecci, Katia Castanho
K1 AP endonuclease
K1 BER
K1 Complementation assay
K1 Protein expression
K1 Saccharum spp.
AB Plants are sessile organisms that need to cope with different conditions. The Base Excision Repair (BER) pathway is an important mechanism protecting the genome from DNA lesions. Apurinic/apyrimidinic (AP) endonucleases are key BER enzymes that process AP sites arising either spontaneously or as BER intermediates. In Arabidopsis there are three AP endonucleases: AtARP1, AtAPE1L, and AtAPE2, and in sugarcane two AtARP1 homologues have been identified: ScARP1 and ScARP3. ScARP1 shares 59% sequence identity with Arabidopsis AtARP. Protein modeling of ScARP1 and AtARP1 revealed conserved active sites and metal binding sites. For biochemical characterisation, recombinant ScARP1 protein displayed AP endonuclease activity both in the presence of MnCl2 or MgCl2 and the optimal temperature for its activity was 37 °C. Under these conditions, 3'-exonuclease, 3'-phosphatase, and 3'-phosphodiesteterase activities were not detectable. We also show that ScARP1 protein is able to complement mutant atarp-/- cell extracts deficient in AP endonuclease activity. These results suggest that AP endonucleases from different plant species preserve AP endonuclease activity. The biochemical characterisation of ScARP1 extends our knowledge of the BER pathway to a monocot crop plant group.
PB Elsevier
YR 2019
FD 2019-04-22
LK http://hdl.handle.net/10668/13951
UL http://hdl.handle.net/10668/13951
LA en
NO Cabral Medeiros NM, Córdoba-Cañero D, García-Gil CB, Ariza RR, Roldán-Arjona T, Scortecci KC. Characterization of an AP endonuclease from sugarcane - ScARP1. Biochem Biophys Res Commun. 2019 Jun 30;514(3):926-932
NO This work was funded by a grant from CNPq/Brazil (to K.C.S.) and grants from the Spanish Ministry of Science and Innovation and the European Regional Development Fund BFU2016-80728-P and the Junta de Andalucía and the European Regional Development Fund P11-CVI7576 (to T.R.R). N.M.C.M. was the recipient of a scholarship from CAPES/Brazil, and C.B.G-G. was supported by a fellowship from Junta de Andalucía, Spain.
DS RISalud
RD Apr 18, 2025