RT Journal Article
T1 Dual functionality of the amyloid protein TasA in Bacillus physiology and fitness on the phylloplane
A1 Cámara-Almirón, Jesús
A1 Navarro, Yurena
A1 Díaz-Martínez, Luis
A1 Magno-Pérez-Bryan, María Concepción
A1 Molina-Santiago, Carlos
A1 Pearson, John R.
A1 de Vicente, Antonio
A1 Pérez-García, Alejandro
A1 Romero, Diego
K1 Bacillus subtilis
K1 Bacterial proteins
K1 Bacteria
K1 Amyloidogenic proteins
K1 Cell death
K1 Cell membrane
K1 Proteínas bacterianas
K1 Bacterias
K1 Proteínas amiloidogénicas
K1 Muerte celular
K1 Membrana celular
AB Bacteria can form biofilms that consist of multicellular communities embedded in an extracellular matrix (ECM). In Bacillus subtilis, the main protein component of the ECM is the functional amyloid TasA. Here, we study further the roles played by TasA in B. subtilis physiology and biofilm formation on plant leaves and in vitro. We show that ΔtasA cells exhibit a range of cytological symptoms indicative of excessive cellular stress leading to increased cell death. TasA associates to the detergent-resistant fraction of the cell membrane, and the distribution of the flotillin-like protein FloT is altered in ΔtasA cells. We propose that, in addition to a structural function during ECM assembly and interactions with plants, TasA contributes to the stabilization of membrane dynamics as cells enter stationary phase.
PB Springer Nature
YR 2020
FD 2020
LK http://hdl.handle.net/10668/3676
UL http://hdl.handle.net/10668/3676
LA en
NO Cámara-Almirón J, Navarro Y, Díaz-Martínez L, Magno-Pérez-Bryan MC, Molina-Santiago C, Pearson JR, et al. Dual functionality of the amyloid protein TasA in Bacillus physiology and fitness on the phylloplane. Nat Commun. 2020 Apr 20;11(1):1859
DS RISalud
RD Apr 10, 2025