RT Journal Article
T1 Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens.
A1 Mediavilla-Gradolph, María Concepción
A1 De Toro-Peinado, Inmaculada
A1 Bermúdez-Ruiz, María Pilar
A1 García-Martínez, María de los Ángeles
A1 Ortega-Torres, María
A1 Montiel Quezel-Guerraz, Natalia
A1 Palop-Borrás, Begoña
K1 Humanos
K1 Genotipo
K1 Infecciones por micobacterias no tuberculosas
K1 Micobacterias no tuberculosas
K1 Espectrometría de masas con desorción-ionización láser asistida por matriz
AB The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.
PB Hindawi Publishing Corporation
YR 2015
FD 2015
LK http://hdl.handle.net/10668/2240
UL http://hdl.handle.net/10668/2240
LA en
NO Mediavilla-Gradolph MC, De Toro-Peinado I, Bermúdez-Ruiz MP, García-Martínez Mde L, Ortega-Torres M, Montiel Quezel-Guerraz N, et al. Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens. Biomed Res Int. 2015; 2015:854078
NO Journal Article;
DS RISalud
RD Apr 16, 2025